Literature summary extracted from

Casalone, E.; Barberio, C.; Cavalieri, D.; Polsinelli, M.
Identification by functional analysis of the gene encoding a-isopropylmalate synthase II (LEU9) in Saccharomyces cerevisiae (2000), Yeast, 16, 539-545.

Cloned(Commentary)

EC Number	Cloned (Comment)	Organism
2.3.3.13	construction of three knock-out mutants, two carrying a deletion in YOR108w or in Leu4 ORF, and one carrying both the deletions	Saccharomyces cerevisiae

Natural Substrates/ Products (Substrates)

EC Number	Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
2.3.3.13	2-oxo-3-methylbutanoate + acetyl-CoA + H2O	Saccharomyces cerevisiae	first enzyme in biosynthesis of L-Leu	?	-	?

Organism

EC Number	Organism	UniProt	Comment	Textmining
2.3.3.13	Saccharomyces cerevisiae	-	-	-

Specific Activity [micromol/min/mg]

EC Number	Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
2.3.3.13	additional information	-	-	Saccharomyces cerevisiae

Substrates and Products (Substrate)

EC Number	Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
2.3.3.13	2-oxo-3-methylbutanoate + acetyl-CoA + H2O	-	Saccharomyces cerevisiae	3-hydroxy-4-methyl-3-carboxypentanoate + CoA	i.e. alpha-isopropylmalate	?
2.3.3.13	2-oxo-3-methylbutanoate + acetyl-CoA + H2O	first enzyme in biosynthesis of L-Leu	Saccharomyces cerevisiae	?	-	?

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Release 2023.1 (January 2023)