EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
2.3.3.13 | 5',5',5'-trifluoroleucine | - |
Saccharomyces cerevisiae | |
2.3.3.13 | CoA | - |
Saccharomyces cerevisiae | |
2.3.3.13 | D-leucine amide | weak | Saccharomyces cerevisiae | |
2.3.3.13 | L-2-Hydroxyisopentanoate | - |
Saccharomyces cerevisiae | |
2.3.3.13 | leucine | both Fe2+ and Co2+ lower the inhibition by Leu; D-Leu, weak; mixed-type inhibition, strongly pH-dependent, Leu concentration necessary for half-maximal inhibition increases about 10fold as the pH increases from 7.5 to 8.5 | Saccharomyces cerevisiae |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.3.3.13 | 0.009 | - |
acetyl-CoA | - |
Saccharomyces cerevisiae | |
2.3.3.13 | 0.016 | - |
2-oxo-3-methylbutanoate | - |
Saccharomyces cerevisiae | |
2.3.3.13 | 0.57 | - |
2-oxobutanoate | - |
Saccharomyces cerevisiae |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
2.3.3.13 | K+ | required | Saccharomyces cerevisiae |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
2.3.3.13 | 121000 | - |
in absence of Leu, gel filtration | Saccharomyces cerevisiae |
2.3.3.13 | 137000 | - |
in presence of substrate, gel filtration | Saccharomyces cerevisiae |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.3.3.13 | 2-oxo-3-methylbutanoate + acetyl-CoA + H2O | Saccharomyces cerevisiae | first enzyme in biosynthesis of L-Leu | ? | - |
? | |
2.3.3.13 | 2-oxo-3-methylbutanoate + acetyl-CoA + H2O | Saccharomyces cerevisiae 60615 | first enzyme in biosynthesis of L-Leu | ? | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.3.3.13 | Saccharomyces cerevisiae | - |
- |
- |
2.3.3.13 | Saccharomyces cerevisiae 60615 | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
2.3.3.13 | - |
Saccharomyces cerevisiae |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
2.3.3.13 | 1.58 | - |
- |
Saccharomyces cerevisiae |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.3.3.13 | 2-oxo-3-methylbutanoate + acetyl-CoA + H2O | - |
Saccharomyces cerevisiae | 3-hydroxy-4-methyl-3-carboxypentanoate + CoA | i.e. alpha-isopropylmalate | ? | |
2.3.3.13 | 2-oxo-3-methylbutanoate + acetyl-CoA + H2O | - |
Saccharomyces cerevisiae 60615 | 3-hydroxy-4-methyl-3-carboxypentanoate + CoA | i.e. alpha-isopropylmalate | ? | |
2.3.3.13 | 2-oxo-3-methylbutanoate + acetyl-CoA + H2O | first enzyme in biosynthesis of L-Leu | Saccharomyces cerevisiae | ? | - |
? | |
2.3.3.13 | 2-oxo-3-methylbutanoate + acetyl-CoA + H2O | first enzyme in biosynthesis of L-Leu | Saccharomyces cerevisiae 60615 | ? | - |
? | |
2.3.3.13 | 2-oxo-butanoate + acetyl-CoA + H2O | - |
Saccharomyces cerevisiae | 2-ethyl-2-hydroxysuccinic acid + CoA | - |
? | |
2.3.3.13 | 2-oxo-butanoate + acetyl-CoA + H2O | - |
Saccharomyces cerevisiae 60615 | 2-ethyl-2-hydroxysuccinic acid + CoA | - |
? | |
2.3.3.13 | pyruvate + acetyl-CoA + H2O | - |
Saccharomyces cerevisiae | 2-hydroxy-2-methylsuccinic acid + CoA | - |
? | |
2.3.3.13 | pyruvate + acetyl-CoA + H2O | - |
Saccharomyces cerevisiae 60615 | 2-hydroxy-2-methylsuccinic acid + CoA | - |
? |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
2.3.3.13 | additional information | - |
Leu, and to a lesser extent, 2-oxo-3-methylbutanoate stabilize against heat inactivation, acetyl-CoA accelerates inactivation | Saccharomyces cerevisiae |
2.3.3.13 | 50 | - |
pH 6.9, 5 min, 80% loss of activity, 1 mM Leu provides significant but incomplete protection | Saccharomyces cerevisiae |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
2.3.3.13 | 8 | - |
- |
Saccharomyces cerevisiae |