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Literature summary extracted from

  • Tracy, J.W.; Kohlhaw, G.B.
    Evidence for two distinct CoA binding sites on yeast alpha-isopropylmalate synthase (1977), J. Biol. Chem., 252, 4085-4091.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.3.3.13 CoA two distinct CoA sites on each enzyme subunit: the first site interacts with CoA an desulfo-CoA, the second site is absolutely specific for CoA. Binding of CoA to this site occurs only when Zn2+ is present, is independent of the specific activity of the enzyme and does not eliminate CoA binding at the product site; Zn2+-dependent reversible inactivation Saccharomyces cerevisiae
2.3.3.13 desulfo-CoA
-
Saccharomyces cerevisiae

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.3.3.13 65000
-
x * 65000, equilibrium sedimentation under denaturing conditions Saccharomyces cerevisiae
2.3.3.13 67000
-
2 * 67000, SDS-PAGE Saccharomyces cerevisiae
2.3.3.13 127000
-
sedimentation equilibrium centrifugation Saccharomyces cerevisiae

Organism

EC Number Organism UniProt Comment Textmining
2.3.3.13 Saccharomyces cerevisiae
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.3.13
-
Saccharomyces cerevisiae

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.3.3.13 7.1
-
-
Saccharomyces cerevisiae

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.3.13 2-oxo-3-methylbutanoate + acetyl-CoA + H2O
-
Saccharomyces cerevisiae 3-hydroxy-4-methyl-3-carboxypentanoate + CoA i.e. alpha-isopropylmalate ?

Subunits

EC Number Subunits Comment Organism
2.3.3.13 ? x * 65000, equilibrium sedimentation under denaturing conditions Saccharomyces cerevisiae
2.3.3.13 dimer 2 * 67000, SDS-PAGE Saccharomyces cerevisiae

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.3.3.13 0.07
-
CoA with respect to acetyl-CoA Saccharomyces cerevisiae
2.3.3.13 0.09
-
desulfo-CoA with respect to acetyl-CoA Saccharomyces cerevisiae