Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Wiegel, J.; Schlegel, H.G.
    alpha-Isopropylmalate synthase from Alcaligenes eutrophus H 16. III. Endproduct inhibition and its relief by valine and isoleucine (1977), Arch. Microbiol., 114, 203-210.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.3.3.13 2-Oxo-isohexanoate
-
Cupriavidus necator
2.3.3.13 leucine at low concentrations of Leu the inhibition mechanism is of the competitive type with respect to substrate acetyl-CoA and of the non-competitive type with respect to 2-oxo-3-methylbutanoate; L-Leu Cupriavidus necator

Organism

EC Number Organism UniProt Comment Textmining
2.3.3.13 Cupriavidus necator
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.3.13 2-oxo-3-methylbutanoate + acetyl-CoA + H2O
-
Cupriavidus necator 3-hydroxy-4-methyl-3-carboxypentanoate + CoA
-
?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.3.3.13 44
-
pH: 7.5, temperature optimum increased to 45°C in the presence of 2 mM valine and to 48°C in the presence of leucine Cupriavidus necator

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.3.3.13 8.5
-
-
Cupriavidus necator

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.3.3.13 0.001
-
leucine pH 7.35, acetyl-CoA varied Cupriavidus necator
2.3.3.13 0.002
-
leucine pH 7.6, acetyl-CoA varied Cupriavidus necator
2.3.3.13 0.0025
-
leucine pH 8.1, acetyl-CoA varied Cupriavidus necator
2.3.3.13 0.1
-
leucine pH 7.6, 2-oxo-3-methylbutanoate varied Cupriavidus necator
2.3.3.13 0.1
-
2-Oxo-isohexanoate with respect to 2-oxo-3-methylbutanoate Cupriavidus necator
2.3.3.13 0.2
-
leucine pH 8.1, 2-oxo-3-methylbutanoate varied Cupriavidus necator
2.3.3.13 0.2
-
2-Oxo-isohexanoate with respect to acetyl-CoA Cupriavidus necator