EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
2.3.3.13 | K2SO4 | 250 mM K2SO4, pH 7.5, 20% inhibition | Cupriavidus necator | |
2.3.3.13 | KCl | 400 mM KCl, pH 8.2, 20% inhibition | Cupriavidus necator |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
2.3.3.13 | Mn2+ | 1 mM, 25% activation | Cupriavidus necator |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
2.3.3.13 | 100000 | - |
in presence of Leu, gel filtration | Cupriavidus necator |
2.3.3.13 | 114500 | - |
in absence of Leu, gel filtration | Cupriavidus necator |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.3.3.13 | 2-oxo-3-methylbutanoate + acetyl-CoA + H2O | Cupriavidus necator | first enzyme in biosynthesis of L-Leu | ? | - |
? | |
2.3.3.13 | 2-oxo-3-methylbutanoate + acetyl-CoA + H2O | Cupriavidus necator H 16 | first enzyme in biosynthesis of L-Leu | ? | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.3.3.13 | Cupriavidus necator | - |
- |
- |
2.3.3.13 | Cupriavidus necator | - |
Val-Ile double auxotrophic mutant of strain H 16 | - |
2.3.3.13 | Cupriavidus necator H 16 | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
2.3.3.13 | - |
Cupriavidus necator |
EC Number | Renatured (Comment) | Organism |
---|---|---|
2.3.3.13 | irreversible denaturation after 5 min at 60°C | Cupriavidus necator |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
2.3.3.13 | additional information | highest specific activity in cells harvested in the late exponential phase | Cupriavidus necator | - |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
2.3.3.13 | 12.05 | - |
- |
Cupriavidus necator |
EC Number | Storage Stability | Organism |
---|---|---|
2.3.3.13 | 30°C, pH 7.5, stable for more than 10 days | Cupriavidus necator |
2.3.3.13 | freezing at -20°C and thawing, about 10% loss of activity. Storage at -20°C, 100 mM potassium phosphate, 0.2 mM 2-oxo-3-methylbutanoate, pH 7.5 or pH 8.0, no further loss of activity after 5 months | Cupriavidus necator |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.3.3.13 | 2-oxo-3-methylbutanoate + acetyl-CoA + H2O | - |
Cupriavidus necator | 3-hydroxy-4-methyl-3-carboxypentanoate + CoA | - |
? | |
2.3.3.13 | 2-oxo-3-methylbutanoate + acetyl-CoA + H2O | - |
Cupriavidus necator H 16 | 3-hydroxy-4-methyl-3-carboxypentanoate + CoA | - |
? | |
2.3.3.13 | 2-oxo-3-methylbutanoate + acetyl-CoA + H2O | first enzyme in biosynthesis of L-Leu | Cupriavidus necator | ? | - |
? | |
2.3.3.13 | 2-oxo-3-methylbutanoate + acetyl-CoA + H2O | first enzyme in biosynthesis of L-Leu | Cupriavidus necator H 16 | ? | - |
? |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
2.3.3.13 | 44 | - |
- |
Cupriavidus necator |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
2.3.3.13 | additional information | - |
cold lability can be overcome by stabilizing the enzyme by buffer of increased ionic strength | Cupriavidus necator |
2.3.3.13 | additional information | - |
stabilization at low temperature with KCl | Cupriavidus necator |
2.3.3.13 | 60 | - |
5 min, in absence of substrates irreversible denaturation | Cupriavidus necator |
2.3.3.13 | 65 | - |
5 min, in presence of 2-oxo-3-methylbutanoate and acetyl-CoA, stable | Cupriavidus necator |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
2.3.3.13 | 8 | 8.5 | Tris-HCl buffer | Cupriavidus necator |
2.3.3.13 | 8.2 | - |
phosphate buffer | Cupriavidus necator |
EC Number | pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|---|
2.3.3.13 | 7 | - |
3 h, about 15% of maximal activity | Cupriavidus necator |
2.3.3.13 | 7.2 | 8.2 | 3 h, stable | Cupriavidus necator |
2.3.3.13 | 9 | - |
3 h, about 50% loss of activity | Cupriavidus necator |
2.3.3.13 | 9.5 | - |
3 h, about 30% of maximal activity | Cupriavidus necator |