Literature summary extracted from

Dupuy, C.; Virion, A.; Hammou, N.A.; Kaniewski, J.; Deme, D.; Pommier, J.
Solubilization and characteristics of the thyroid NADPH-dependent hydrogen peroxide generating system (1986), Biochem. Biophys. Res. Commun., 141, 839-846.

General Stability

EC Number	General Stability	Organism
1.6.3.1	CHAPS is the best detergent for solubilization of enzyme, preserves native properties of enzyme	Sus scrofa

Inhibitors

EC Number	Inhibitors	Comment	Organism	Structure
1.6.3.1	DTNB	-	Sus scrofa
1.6.3.1	EGTA	almost complete inhibition at 0.5 mM	Sus scrofa
1.6.3.1	N-ethylmaleimide	weak inhibition	Sus scrofa
1.6.3.1	p-hydroxymercuribenzoate	-	Sus scrofa

Localization

EC Number	Localization	Comment	Organism	GeneOntology No.	Textmining
1.6.3.1	apical plasma membrane	-	Sus scrofa	16324	-

Metals/Ions

EC Number	Metals/Ions	Comment	Organism	Structure
1.6.3.1	Ca2+	required for full activity	Sus scrofa
1.6.3.1	Ca2+	regulates enzyme activity	Sus scrofa
1.6.3.1	Cd2+	can replace for Ca2+	Sus scrofa
1.6.3.1	additional information	Mg2+ has no effect	Sus scrofa
1.6.3.1	Zn2+	can replace for Ca2+	Sus scrofa

Natural Substrates/ Products (Substrates)

EC Number	Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
1.6.3.1	NAD(P)H + H+ + O2	Sus scrofa	production of hydrogen peroxide in thyroid gland that is required for thyroid hormone synthesis	NAD(P)+ + H2O2	-	?

Organism

EC Number	Organism	UniProt	Comment	Textmining
1.6.3.1	Sus scrofa	-	-	-

Source Tissue

EC Number	Source Tissue	Comment	Organism	Textmining
1.6.3.1	thyroid gland	-	Sus scrofa	-

Substrates and Products (Substrate)

EC Number	Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
1.6.3.1	NAD(P)H + H+ + O2	-	Sus scrofa	NAD(P)+ + H2O2	-	?
1.6.3.1	NAD(P)H + H+ + O2	production of hydrogen peroxide in thyroid gland that is required for thyroid hormone synthesis	Sus scrofa	NAD(P)+ + H2O2	-	?

pH Optimum

EC Number	pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
1.6.3.1	6	8	-	Sus scrofa

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Release 2023.1 (January 2023)