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Literature summary extracted from

  • Kretovich, W.L.; Stepanovich, K.M.
    The enzyme catalyzing hydroxypyruvate reductive amination (1966), Izv. Akad. Nauk SSSR Ser. Biol., 2, 295-301.
No PubMed abstract available

General Stability

EC Number General Stability Organism
1.4.1.7 very labile enzyme, loses its activity when exposed to dialysis or weak heating Petroselinum crispum

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.4.1.7 p-chloromercurybenzoate completely inhibited at 0.005 mM Petroselinum crispum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.4.1.7 0.0005
-
 Hydroxypyruvate
-
 Petroselinum crispum
1.4.1.7 0.015
-
 NH3
-
 Petroselinum crispum

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.4.1.7 L-serine + H2O + NAD+ Petroselinum crispum
-
 3-hydroxypyruvate + NH3 + NADH
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.4.1.7 Petroselinum crispum
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.4.1.7 isolated from an albumin fraction, purified on Sephadex G-76, subsequently on DEAE-cellulose Petroselinum crispum

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.4.1.7 leaf green leaves Petroselinum crispum
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.4.1.7 L-serine + H2O + NAD+
-
 Petroselinum crispum 3-hydroxypyruvate + NH3 + NADH
-
 ?
1.4.1.7 additional information no substrates in presence of NADH: alpha-ketoglutarate, oxaloacetate, glyoxylate, or pyruvate Petroselinum crispum ?
-
 ?

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.4.1.7 10
-
 in phosphate buffer Petroselinum crispum

Cofactor

EC Number Cofactor Comment Organism Structure
1.4.1.7 NADH specific for NADH, does not react with NADPH, strictly specific for hydroxypyruvate in presence of NADH Petroselinum crispum