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Literature summary extracted from

  • Mano, J.i.; Torii, Y.; Hayashi, S.i.; Takimoto, K.; Matsui, K.; Nakamura, K.; Inze, D.; Babiychuk, E.; Kushnir, S.; Asada, K.
    The NADPH:quinone oxidoreductase P1-x-crystallin in Arabidopsis catalyzes the alpha,beta-hydrogenation of 2-alkenals: Detoxication of the lipid peroxide-derived reactive aldehydes (2002), Plant Cell Physiol., 43, 1445-1455.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.3.1.74
-
 Arabidopsis thaliana

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.3.1.74 0.005
-
 (2E)-nonenal pH 6 Arabidopsis thaliana
1.3.1.74 0.0052
-
 3-penten-2-one pH 6 Arabidopsis thaliana
1.3.1.74 0.0134
-
 4-hydroxy-(2E)-nonenal pH 6 Arabidopsis thaliana
1.3.1.74 0.055
-
 3-buten-2-one pH 6 Arabidopsis thaliana
1.3.1.74 0.128
-
 Diamide pH 6 Arabidopsis thaliana
1.3.1.74 0.145
-
 4-hydroxy-(2E)-hexenal pH 6 Arabidopsis thaliana
1.3.1.74 0.232
-
 (2E)-hexenal pH 6 Arabidopsis thaliana
1.3.1.74 1.42
-
 (2E)-pentenal pH 6 Arabidopsis thaliana
1.3.1.74 4.65
-
 propenal pH 6 Arabidopsis thaliana

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.3.1.74 n-alkanal + NAD(P)+ Arabidopsis thaliana role in detoxication alk-2-enal + NAD(P)H
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.3.1.74 Arabidopsis thaliana
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.3.1.74 homogeneity Arabidopsis thaliana

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.3.1.74 (2E)-hexenal + NAD(P)H
-
 Arabidopsis thaliana n-hexanal + NAD(P)+
-
 ?
1.3.1.74 (2E)-nonenal + NAD(P)H
-
 Arabidopsis thaliana n-nonanal + NAD(P)+
-
 ?
1.3.1.74 (2E)-pentenal + NAD(P)H
-
 Arabidopsis thaliana n-pentanal + NAD(P)+
-
 ?
1.3.1.74 3-buten-2-one + NAD(P)H
-
 Arabidopsis thaliana 2-butanone + NAD(P)+
-
 ?
1.3.1.74 3-penten-2-one + NAD(P)H
-
 Arabidopsis thaliana 2-pentanone + NAD(P)+
-
 ?
1.3.1.74 4-hydroxy-(2E)-hexenal + NAD(P)H
-
 Arabidopsis thaliana 4-hydroxyhexenal + NAD(P)+
-
 ?
1.3.1.74 4-hydroxy-(2E)-nonenal + NAD(P)H
-
 Arabidopsis thaliana 4-hydroxynonylaldehyde + NAD(P)+
-
 ?
1.3.1.74 diamide + NAD(P)H
-
 Arabidopsis thaliana ? + NAD(P)+
-
 ?
1.3.1.74 additional information catalyzes the hydrogenation of alpha,beta-unsaturated bonds, but not the reduction of the aldehyde moiety in 2-alkenal molecules Arabidopsis thaliana ?
-
 ?
1.3.1.74 n-alkanal + NAD(P)+ carbon chain length C3-C9 serves as substrates Arabidopsis thaliana alk-2-enal + NAD(P)H
-
 ?
1.3.1.74 n-alkanal + NAD(P)+ role in detoxication Arabidopsis thaliana alk-2-enal + NAD(P)H
-
 ?
1.3.1.74 propenal + NAD(P)H synonym: acrolein Arabidopsis thaliana propanal + NAD(P)+
-
 ?

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.3.1.74 0.583
-
 (2E)-pentenal pH 6 Arabidopsis thaliana
1.3.1.74 0.667
-
 propenal pH 6 Arabidopsis thaliana
1.3.1.74 0.7
-
 4-hydroxy-(2E)-hexenal pH 6 Arabidopsis thaliana
1.3.1.74 0.85
-
 (2E)-nonenal pH 6 Arabidopsis thaliana
1.3.1.74 1.05
-
 (2E)-hexenal pH 6 Arabidopsis thaliana
1.3.1.74 1.38
-
 3-buten-2-one pH 6 Arabidopsis thaliana
1.3.1.74 1.47
-
 4-hydroxy-(2E)-nonenal pH 6 Arabidopsis thaliana
1.3.1.74 1.58
-
 Diamide pH 6 Arabidopsis thaliana
1.3.1.74 1.72
-
 3-penten-2-one pH 6 Arabidopsis thaliana

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.3.1.74 6
-
-
 Arabidopsis thaliana

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.3.1.74 5.3 6.6 50% activity at pH 5.3 and pH 6.6 Arabidopsis thaliana

Cofactor

EC Number Cofactor Comment Organism Structure
1.3.1.74 NADPH
-
 Arabidopsis thaliana