EC Number | Activating Compound | Comment | Organism | Structure |
---|---|---|---|---|
1.21.4.2 | arsenate | absolutely required for protein C activity | Acetoanaerobium sticklandii |
EC Number | General Stability | Organism |
---|---|---|
1.21.4.2 | the ability of protein component C to catalyse the arsenate-dependent decomposition of acetyl phosphate is inhibited by alkylation selenoprotein A alkylated at pH 6 with bromoacetate is active as a component of the enzyme complex | Acetoanaerobium sticklandii |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
1.21.4.2 | (NH4)2SO4 | inhibits the ability of protein component C to catalyse the arsenate-dependent decomposition of acetyl phosphate | Acetoanaerobium sticklandii | |
1.21.4.2 | Bromoacetate | 75Se-labeled protein A preparation is inactivated at pH 6, 25°C, for 10 min in presence of 10 mM bromoacetate by about 25% | Acetoanaerobium sticklandii | |
1.21.4.2 | iodoacetate | the ability of protein component C to catalyse the arsenate-dependent decomposition of acetyl phosphate is inhibited, but protein C is protected from inactivation by treatment acetyl phosphate | Acetoanaerobium sticklandii | |
1.21.4.2 | KCl | the ability of protein component C to catalyse the arsenate-dependent decomposition of acetyl phosphate is inhibited | Acetoanaerobium sticklandii |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.21.4.2 | acetyl phosphate + NH3 + thioredoxin disulfide + H2O | Acetoanaerobium sticklandii | - |
glycine + phosphate + thioredoxin | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.21.4.2 | Acetoanaerobium sticklandii | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.21.4.2 | of protein C of enzyme | Acetoanaerobium sticklandii |
EC Number | Reaction | Comment | Organism | Reaction ID |
---|---|---|---|---|
1.21.4.2 | acetyl phosphate + NH3 + thioredoxin disulfide + H2O = glycine + phosphate + thioredoxin | the protein C component catalyses the arsenate-dependent decomposition of acetyl phosphate | Acetoanaerobium sticklandii | |
1.21.4.2 | acetyl phosphate + NH3 + thioredoxin disulfide + H2O = glycine + phosphate + thioredoxin | The reaction is observed only in the direction of glycine reduction. The enzyme consists of three protein components A, B and C. Protein B contains selenocysteine and a pyruvoyl group, and is responsible for glycine binding and ammonia release. Protein A, which also contains selenocysteine, is reduced by thioredoxin, and is needed to convert the carboxymethyl group into a ketene equivalent, in turn used by protein C to produce acetyl phosphate. Only protein B distinguishes this enzyme from EC 1.21.4.3 (sarcosine reductase) and EC 1.21.4.4 (betaine reductase) | Acetoanaerobium sticklandii |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.21.4.2 | acetyl phosphate + NH3 + thioredoxin disulfide + H2O | - |
Acetoanaerobium sticklandii | glycine + phosphate + thioredoxin | - |
? | |
1.21.4.2 | additional information | Se-carboxymethyl selenprotein A is a substrate of protein C | Acetoanaerobium sticklandii | ? | - |
? |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.21.4.2 | 47 | - |
30% loss of activity of protein C after heating for 10 min, at pH 7.0, in the presence of EDTA, with and without Mg2+ | Acetoanaerobium sticklandii |
1.21.4.2 | 68 | - |
80% loss of activity of protein C after heating for 10 min, at pH 7.0, in the presence of EDTA, with and without Mg2+ | Acetoanaerobium sticklandii |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
1.21.4.2 | additional information | - |
pI of protein component C: 5.7 | Acetoanaerobium sticklandii |