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Literature summary extracted from

  • Wagner, M.; Sonntag, D.; Grimm, R.; Pich, A.; Eckerskorn, C.; Söhling, B.; Andreesen, J.R.
    Substrate-specific selenoprotein B of glycine reductase from Eubacterium acidaminophilum (1999), Eur. J. Biochem., 260, 38-49.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.21.4.2 cloning and sequencing of a new gene region, encoding a proprotein for the beta and alpha subunits of selenoprotein B: grdE, selenoprotein A: grdA and selenium-containing gamma subunit of selenoprotein B: grdB Peptoclostridium acidaminophilum

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.21.4.2 22000
-
 selenoprotein B, alpha,beta,gamma, 2 * 22000 + 2 * 25000 + 2 * 47000 Peptoclostridium acidaminophilum
1.21.4.2 25000
-
 selenoprotein B, alpha,beta,gamma, 2 * 22000 + 2 * 25000 + 2 * 47000 Peptoclostridium acidaminophilum
1.21.4.2 47000
-
 selenoprotein B, alpha,beta,gamma, 2 * 22000 + 2 * 25000 + 2 * 47000 Peptoclostridium acidaminophilum

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.21.4.2 acetyl phosphate + NH3 + thioredoxin disulfide + H2O Peptoclostridium acidaminophilum
-
 glycine + phosphate + thioredoxin
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.21.4.2 Peptoclostridium acidaminophilum Q9R4G7 DSM 5388T, growth on serine
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.21.4.2 of selenoprotein B of enzyme Peptoclostridium acidaminophilum

Reaction

EC Number Reaction Comment Organism Reaction ID
1.21.4.2 acetyl phosphate + NH3 + thioredoxin disulfide + H2O = glycine + phosphate + thioredoxin mechanism Peptoclostridium acidaminophilum
a
1.21.4.2 acetyl phosphate + NH3 + thioredoxin disulfide + H2O = glycine + phosphate + thioredoxin cleavage mechanism for the pyruvoyl group dependent reductase starting from cysteine Peptoclostridium acidaminophilum
a
1.21.4.2 acetyl phosphate + NH3 + thioredoxin disulfide + H2O = glycine + phosphate + thioredoxin The reaction is observed only in the direction of glycine reduction. The enzyme consists of three protein components A, B and C. Protein B contains selenocysteine and a pyruvoyl group, and is responsible for glycine binding and ammonia release. Protein A, which also contains selenocysteine, is reduced by thioredoxin, and is needed to convert the carboxymethyl group into a ketene equivalent, in turn used by protein C to produce acetyl phosphate. Only protein B distinguishes this enzyme from EC 1.21.4.3 (sarcosine reductase) and EC 1.21.4.4 (betaine reductase) Peptoclostridium acidaminophilum
a

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.21.4.2 352
-
 substrate-specific selenoprotein B of enzyme Peptoclostridium acidaminophilum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.21.4.2 acetyl phosphate + NH3 + thioredoxin disulfide + H2O
-
 Peptoclostridium acidaminophilum glycine + phosphate + thioredoxin
-
 ?

Subunits

EC Number Subunits Comment Organism
1.21.4.2 hexamer selenoprotein B, alpha,beta,gamma, 2 * 22000 + 2 * 25000 + 2 * 47000 Peptoclostridium acidaminophilum