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Literature summary extracted from

  • Kong, K.H.; Hong, M.P.; Choi, S.S.; Kim, Y.T.; Cho, S.H.
    Purification and characterization of a highly stable tyrosinase from Thermomicrobium roseum (2000), Biotechnol. Appl. Biochem., 31, 113-118.
No PubMed abstract available

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.14.18.1 2-mercaptoethanol 0.1 mM, complete inhibition Thermomicrobium roseum
1.14.18.1 5-hydroxy-2-(hydroxymethyl)-2H-pyran-4-one 1 mM, 50% inhibition of L-dopa oxidation Thermomicrobium roseum
1.14.18.1 Cl- 1 mM, 35% inhibition of L-dopa oxidation Thermomicrobium roseum
1.14.18.1 diethyldithiocarbamate 1 mM, complete inhibition of L-dopa oxidation Thermomicrobium roseum
1.14.18.1 EDTA 1 mM, 71% inhibition of L-dopa oxidation Thermomicrobium roseum
1.14.18.1 glutathione 1 mM, 86% inhibition of L-dopa oxidation Thermomicrobium roseum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.14.18.1 0.18
-
 L-Dopa
-
 Thermomicrobium roseum

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.14.18.1 copper 50% activation of L-dopa oxidation Thermomicrobium roseum
1.14.18.1 K+ 50% activation of L-dopa oxidation Thermomicrobium roseum
1.14.18.1 Mg2+ 33% activation of L-dopa oxidation Thermomicrobium roseum

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.14.18.1 43000
-
 2 * 43000, SDS-PAGE Thermomicrobium roseum
1.14.18.1 90000
-
 gel filtration Thermomicrobium roseum

Organism

EC Number Organism UniProt Comment Textmining
1.14.18.1 Thermomicrobium roseum
-
 ATCC 27502
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.14.18.1 DEAE-Sephacel, Mono Q Thermomicrobium roseum

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.14.18.1 2.49
-
-
 Thermomicrobium roseum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.18.1 (RS)-catechin + O2 83% of activity with L-dopa Thermomicrobium roseum ?
-
 ?
1.14.18.1 4-hydroxybenzoic acid + O2 + AH2 50% of activity with L-dopa Thermomicrobium roseum 3,4-dihydroxybenzoic acid + H2O + A
-
 ?
1.14.18.1 caffeic acid + 1/2 O2 17% of activity with L-dopa Thermomicrobium roseum caffeoyl quinone + H2O
-
 ?
1.14.18.1 catechol + 1/2 O2 125% of activity with L-dopa Thermomicrobium roseum 1,2-benzoquinone + H2O
-
 ?
1.14.18.1 chlorogenic acid + O2 108% of activity with L-dopa Thermomicrobium roseum chlorogenoquinone + H2O
-
 ?
1.14.18.1 D-catechin + O2 67% of activity with L-dopa Thermomicrobium roseum ?
-
 ?
1.14.18.1 DL-epicatechin + O2 91% of activity with L-dopa Thermomicrobium roseum ?
-
 ?
1.14.18.1 dopa + 1/2 O2
-
 Thermomicrobium roseum dopaquinone + H2O
-
 ?
1.14.18.1 L-tyrosine + O2 + AH2 10% of activity with L-dopa Thermomicrobium roseum L-dopa + H2O + A
-
 ?
1.14.18.1 o-coumaric acid + O2 58% of activity with L-dopa Thermomicrobium roseum ?
-
 ?
1.14.18.1 orcin + O2 42% of activity with L-dopa Thermomicrobium roseum ?
-
 ?
1.14.18.1 phloroglucin + O2 87% of activity with L-dopa Thermomicrobium roseum ?
-
 ?
1.14.18.1 pyrogallol + O2 417% of activity with L-dopa Thermomicrobium roseum ?
-
 ?
1.14.18.1 resorcinol + O2 67% of activity with L-dopa Thermomicrobium roseum ?
-
 ?
1.14.18.1 tyramine + O2 67% of activity with L-dopa Thermomicrobium roseum 4-(2-aminoethyl)cyclohexa-3,5-diene-1,2-dione + H2O
-
 ?

Subunits

EC Number Subunits Comment Organism
1.14.18.1 dimer 2 * 43000, SDS-PAGE Thermomicrobium roseum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.14.18.1 9.5
-
-
 Thermomicrobium roseum

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.14.18.1 7.5 10.5 less than 50% activity below pH 7.5 and above pH 10.5, L-dopa oxidation at 50°C Thermomicrobium roseum

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
1.14.18.1 6
-
 4°C, 20 h, about 75% loss of activity below Thermomicrobium roseum
1.14.18.1 8.5 10 4°C, 20 h, enzyme retains more than 70% of activity in the pH-range 8.5-10.0 Thermomicrobium roseum
1.14.18.1 11
-
 4°C, 20 h, about 75% loss of activity above Thermomicrobium roseum