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Literature summary extracted from
- Expression and characterization of human tyrosinase from a bacterial expression system (2000), Comp. Biochem. Physiol. B, 125, 563-569.
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 1.14.18.1 | expression in Escherichia coli | Homo sapiens |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.14.18.1 | 5-hydroxy-2-(hydroxymethyl)-2H-pyran-4-one | 1.12 mM, 50% inhibition of recombinant enzyme | Homo sapiens |  |
| 1.14.18.1 | arbutin | 3.7 mM, 50% inhibition of recombinant enzyme | Homo sapiens | |
| 1.14.18.1 | glutathione | 0.01 mM, 50% inhibition of recombinant enzyme | Homo sapiens | |
| 1.14.18.1 | L-phenylalanine | 8.34 mM, 50% inhibition of recombinant enzyme | Homo sapiens | |
| 1.14.18.1 | Lactic acid | 3.73 mM, 50% inhibition of recombinant enzyme | Homo sapiens | |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.14.18.1 | 0.17 | - |
L-tyrosine | recombinant enzyme | Homo sapiens | |
| 1.14.18.1 | 0.36 | - |
L-Dopa | recombinant enzyme | Homo sapiens | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 1.14.18.1 | 66000 | - |
x * 66000, recombinant enzyme, SDS-PAGE | Homo sapiens |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.14.18.1 | Homo sapiens | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 1.14.18.1 | recombinant His-tagged enzyme, DEAE-Sephacel, Ni2+-affinity column | Homo sapiens |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 1.14.18.1 | 26 | - |
recombinant enzyme | Homo sapiens |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 1.14.18.1 | ? | x * 66000, recombinant enzyme, SDS-PAGE | Homo sapiens |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 1.14.18.1 | 50 | - |
L-dopa oxidation, recombinant enzyme | Homo sapiens |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 1.14.18.1 | 7.5 | - |
- |
Homo sapiens |
pH Range
| EC Number | pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|---|
| 1.14.18.1 | 6.5 | 8.5 | less than 50% activity below pH 6.5 and above pH 8.5, L-dopa oxidation, recombinant enzyme | Homo sapiens |
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