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Literature summary extracted from

  • Kong, K.H.; Lee, J.L.; Park, H.J.; Cho, S.H.
    Purification and characterization of the tyrosinase isozymes of pine needles (1998), Biochem. Mol. Biol. Int., 45, 717-724.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.14.18.1 L-ascorbic acid 0.04 mM, complete inhibition Pinus densiflora

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.14.18.1 8.7
-
 L-Dopa isoenzyme 1 Pinus densiflora
1.14.18.1 9.3
-
 L-Dopa isoenzyme 2 Pinus densiflora
1.14.18.1 10
-
 L-Dopa isoenzyme 3 Pinus densiflora

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.14.18.1 copper 1 mM, increase in activity Pinus densiflora
1.14.18.1 Fe3+ 1 mM, increase in activity Pinus densiflora
1.14.18.1 Mg2+ 1 mM, increase in activity Pinus densiflora

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.14.18.1 45000
-
 x * 45000, isoenzyme 3, SDS-PAGE Pinus densiflora
1.14.18.1 55000
-
 x * 55000, isoenzyme 2, SDS-PAGE Pinus densiflora
1.14.18.1 65000
-
 x * 65000, isoenzyme 1, SDS-PAGE Pinus densiflora

Organism

EC Number Organism UniProt Comment Textmining
1.14.18.1 Pinus densiflora
-
 3 isoenzymes
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.14.18.1 Sephacryl S-200, Sephadex G-75, DEAE-Sephacel, isoenzymes 1-3 Pinus densiflora

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.18.1 (RS)-catechin + O2 isoenzymes 1-3, 96, 104, and 168% of activity with L-dopa respectively Pinus densiflora ?
-
 ?
1.14.18.1 3,4-dihydroxybenzoic acid + 1/2 O2 isoenzymes 1-3, 22%, 13%, and 13% of L-dopa activity respectively Pinus densiflora (3,4-dioxocyclohexa-1,5-dien-1-yl)acetic acid + H2O
-
 ?
1.14.18.1 caffeic acid + 1/2 O2
-
 Pinus densiflora caffeoyl quinone + H2O
-
 ?
1.14.18.1 catechol + 1/2 O2
-
 Pinus densiflora 1,2-benzoquinone + H2O
-
 ?
1.14.18.1 chlorogenic acid + O2
-
 Pinus densiflora chlorogenoquinone + H2O
-
 ?
1.14.18.1 D-catechin + O2 isoenzymes 1, 2 and 3, 90%, 86% and 188% of L-dopa activity respectively Pinus densiflora ?
-
 ?
1.14.18.1 dopa + 1/2 O2
-
 Pinus densiflora dopaquinone + H2O
-
 ?
1.14.18.1 L-epicatechin + O2 isoenzyme 1, 150% of L-dopa activity, isoenzyme 2 and 3, 170% and 175% of activity with L-dopa respectively Pinus densiflora ?
-
 ?
1.14.18.1 orcin + O2 isoenzymes 1-3, 41%, 33%, and 25% of activity with L-dopa respectively Pinus densiflora ?
-
 ?
1.14.18.1 p-coumaric acid + O2 + AH2
-
 Pinus densiflora caffeic acid + H2O + A
-
 ?
1.14.18.1 pyrogallol + O2 isoenzymes 1-3, 210%, 263%, and 225% of activity with L-dopa respectively Pinus densiflora ?
-
 ?
1.14.18.1 resorcinol + O2 isoenzymes 1-3, 96%, 129%, and 100% of activity with L-dopa respectively Pinus densiflora ?
-
 ?
1.14.18.1 tyramine + O2 isoenzymes 1, 20% of L-dopa activity, isoenzyme2, 13% of activity with L-dopa, isoenzyme 3, 25% of activity with L-dopa Pinus densiflora 4-(2-aminoethyl)cyclohexa-3,5-diene-1,2-dione + H2O
-
 ?

Subunits

EC Number Subunits Comment Organism
1.14.18.1 ? x * 55000, isoenzyme 2, SDS-PAGE Pinus densiflora
1.14.18.1 ? x * 45000, isoenzyme 3, SDS-PAGE Pinus densiflora
1.14.18.1 ? x * 65000, isoenzyme 1, SDS-PAGE Pinus densiflora

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.14.18.1 55 60 isoenzymes 1-3 Pinus densiflora

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.14.18.1 9 9.6 isoenzymes 1-3 Pinus densiflora