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Literature summary extracted from

  • Kanda, K.; Sato, T.; Ishii, S.; Enei, H.; Ejiri, S.
    Purification and properties of tyrosinase isozymes from the gill of Lentinus edodes fruiting body (1996), Biosci. Biotechnol. Biochem., 60, 1273-1278.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.14.18.1 EDTA
-
 Lentinula edodes
1.14.18.1 kojic acid
-
 Lentinula edodes

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.14.18.1 0.001
-
 p-Cresol isoenzyme A3 Lentinula edodes
1.14.18.1 0.001
-
 p-Cresol isoenzyme B1 Lentinula edodes
1.14.18.1 0.001
-
 p-Cresol isoenzyme B2 Lentinula edodes
1.14.18.1 0.001
-
 p-Cresol isoenzyme B3 Lentinula edodes
1.14.18.1 0.001
-
 p-Cresol isozyme A3, B1, B2, or B3, at pH 6,0 and 30°C Lentinula edodes
1.14.18.1 0.002
-
 p-Cresol isoenzyme A1 Lentinula edodes
1.14.18.1 0.002
-
 p-Cresol isozyme A1, at pH 6.0 and 30°C Lentinula edodes
1.14.18.1 0.003
-
 p-Cresol isoenzyme A2 Lentinula edodes
1.14.18.1 0.003
-
 p-Cresol isozyme A2, at pH 6.0 and 30°C Lentinula edodes
1.14.18.1 0.014
-
 L-tyrosine isoenzyme A2 Lentinula edodes
1.14.18.1 0.014
-
 L-tyrosine isoenzyme A3 Lentinula edodes
1.14.18.1 0.014
-
 catechol isoenzyme A3 Lentinula edodes
1.14.18.1 0.014
-
 L-tyrosine isoenzyme B1 Lentinula edodes
1.14.18.1 0.014
-
 L-tyrosine isoenzyme B2 Lentinula edodes
1.14.18.1 0.014
-
 L-tyrosine isoenzyme B3 Lentinula edodes
1.14.18.1 0.014
-
 catechol isozyme A3, at pH 6.0 and 30°C Lentinula edodes
1.14.18.1 0.014
-
 L-tyrosine isozyme A2, A3, B1, B2, or B3, at pH 6,0 and 30°C Lentinula edodes
1.14.18.1 0.015
-
 L-tyrosine isoenzyme A1 Lentinula edodes
1.14.18.1 0.015
-
 L-tyrosine isozyme A1, at pH 6.0 and 30°C Lentinula edodes
1.14.18.1 0.018
-
 catechol isoenzyme B3 Lentinula edodes
1.14.18.1 0.018
-
 catechol isozyme B3, at pH 6.0 and 30°C Lentinula edodes
1.14.18.1 0.074
-
 L-Dopa isoenzyme B3 Lentinula edodes
1.14.18.1 0.074
-
 L-Dopa isozyme B3, at pH 6.0 and 30°C Lentinula edodes
1.14.18.1 0.085
-
 L-Dopa isoenzyme A3 Lentinula edodes
1.14.18.1 0.085
-
 L-Dopa isozyme A3, at pH 6.0 and 30°C Lentinula edodes
1.14.18.1 0.087
-
 catechol isoenzyme B1 Lentinula edodes
1.14.18.1 0.087
-
 catechol isozyme B1, at pH 6.0 and 30°C Lentinula edodes
1.14.18.1 0.089
-
 L-Dopa isoenzyme A2 Lentinula edodes
1.14.18.1 0.089
-
 L-Dopa isozyme A2, at pH 6.0 and 30°C Lentinula edodes
1.14.18.1 0.09
-
 catechol isoenzyme A1 Lentinula edodes
1.14.18.1 0.09
-
 catechol isozyme A1, at pH 6.0 and 30°C Lentinula edodes
1.14.18.1 0.091
-
 catechol isoenzyme A2 Lentinula edodes
1.14.18.1 0.091
-
 catechol isozyme A2, at pH 6.0 and 30°C Lentinula edodes
1.14.18.1 0.104
-
 catechol isoenzyme B2 Lentinula edodes
1.14.18.1 0.104
-
 catechol isozyme B2, at pH 6.0 and 30°C Lentinula edodes
1.14.18.1 0.185
-
 L-Dopa isoenzyme B2 Lentinula edodes
1.14.18.1 0.185
-
 L-Dopa isozyme B2, at pH 6.0 and 30°C Lentinula edodes
1.14.18.1 1.2
-
 L-Dopa isoenzyme B1 Lentinula edodes
1.14.18.1 1.2
-
 L-Dopa isozyme B1, at pH 6.0 and 30°C Lentinula edodes
1.14.18.1 2.2
-
 L-Dopa isoenzyme A1 Lentinula edodes
1.14.18.1 2.2
-
 L-Dopa isozyme A1, at pH 6.0 and 30°C Lentinula edodes

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.14.18.1 15000
-
 x * 55000 + x * 15000, isoenzyme B3, SDS-PAGE Lentinula edodes
1.14.18.1 15000
-
 x * 54000 + x * 15000, isoenzyme A3, SDS-PAGE Lentinula edodes
1.14.18.1 49000
-
 x * 54000 + x * 49000, isoenzymes A1 and A2, SDS-PAGE Lentinula edodes
1.14.18.1 50000
-
 x * 55000 + x * 50000, isoenzymes B1 and B2, SDS-PAGE Lentinula edodes
1.14.18.1 54000
-
 x * 54000 + x * 49000, isoenzymes A1 and A2, SDS-PAGE Lentinula edodes
1.14.18.1 54000
-
 x * 54000 + x * 15000, isoenzyme A3, SDS-PAGE Lentinula edodes
1.14.18.1 55000
-
 x * 55000 + x * 50000, isoenzymes B1 and B2, SDS-PAGE Lentinula edodes
1.14.18.1 55000
-
 x * 55000 + x * 15000, isoenzyme B3, SDS-PAGE Lentinula edodes

Organism

EC Number Organism UniProt Comment Textmining
1.14.18.1 Lentinula edodes
-
 6 isoenzymes
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.14.18.1 protamine sulfate, ammonium sulfate, Superdex 75, DEAE-Sephacel, superdex 75, Q-Sepharose, isoenzymes A1-A3 and B1-B3 Lentinula edodes

Source Tissue

EC Number Source Tissue Comment Organism Textmining

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.18.1 2 catechol + O2
-
 Lentinula edodes 2 1,2-benzoquinone + 2 H2O
-
 r
1.14.18.1 L-DOPA + O2
-
 Lentinula edodes dopaquinone + H2O
-
 r
1.14.18.1 L-tyrosine + O2
-
 Lentinula edodes L-DOPA + H2O
-
 r
1.14.18.1 p-cresol + O2
-
 Lentinula edodes ?
-
 r

Subunits

EC Number Subunits Comment Organism
1.14.18.1 ? x * 55000 + x * 50000, isoenzymes B1 and B2, SDS-PAGE Lentinula edodes
1.14.18.1 ? x * 54000 + x * 49000, isoenzymes A1 and A2, SDS-PAGE Lentinula edodes
1.14.18.1 ? x * 55000 + x * 15000, isoenzyme B3, SDS-PAGE Lentinula edodes
1.14.18.1 ? x * 54000 + x * 15000, isoenzyme A3, SDS-PAGE Lentinula edodes

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.14.18.1 6
-
 isoenzymes A3 and B3 Lentinula edodes
1.14.18.1 6.5
-
 isoenzymes A1, A2, B1 and B2 Lentinula edodes

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
1.14.18.1 0.001
-
 kojic acid isoenzyme A1 Lentinula edodes
1.14.18.1 0.001
-
 kojic acid isoenzyme B3 Lentinula edodes
1.14.18.1 0.003
-
 kojic acid isoenzyme A3 Lentinula edodes
1.14.18.1 0.003
-
 kojic acid isoenzyme B1 Lentinula edodes
1.14.18.1 0.006
-
 kojic acid isoenzyme A2 Lentinula edodes
1.14.18.1 0.007
-
 EDTA isoenzyme A3 Lentinula edodes
1.14.18.1 0.008
-
 kojic acid isoenzyme B2 Lentinula edodes
1.14.18.1 0.075
-
 EDTA isoenzyme B3 Lentinula edodes
1.14.18.1 0.298
-
 EDTA isoenzyme A1 Lentinula edodes
1.14.18.1 0.58
-
 EDTA isoenzyme B1 Lentinula edodes
1.14.18.1 0.668
-
 EDTA isoenzyme A2 Lentinula edodes
1.14.18.1 1.39
-
 EDTA isoenzyme B2 Lentinula edodes