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Literature summary extracted from
- Kinetic studies on the hydroxylation of p-coumaric acid to caffeic acid by spinach-beet phenolase (1975), Biochem. J., 149, 447-461.
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.14.18.1 | 3,4-dihydroxybenzoic acid | - |
Beta vulgaris |  |
| 1.14.18.1 | Bathocuproine sulfonate | - |
Beta vulgaris | |
| 1.14.18.1 | benzoic acid | - |
Beta vulgaris | |
| 1.14.18.1 | CO | - |
Beta vulgaris | |
| 1.14.18.1 | diethyldithiocarbamate | - |
Beta vulgaris | |
| 1.14.18.1 | p-hydroxybenzoic acid | - |
Beta vulgaris | |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.14.18.1 | 0.067 | - |
O2 | electron donor ascorbic acid | Beta vulgaris | |
| 1.14.18.1 | 0.133 | - |
ascorbic acid | electron donor ascorbic acid | Beta vulgaris | |
| 1.14.18.1 | 0.268 | - |
p-coumaric acid | electron donor ascorbic acid | Beta vulgaris | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.14.18.1 | Beta vulgaris | - |
spinach-beet | - |
Reaction
| EC Number | Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|---|
| 1.14.18.1 | L-tyrosine + O2 = dopaquinone + H2O | 2 o-diphenol + O2 = 2 o-quinone + 2 H2O i.e. catecholase activity, proposed mechanism for sequence of hydroxylase and catecholase | Beta vulgaris | |
| 1.14.18.1 | L-tyrosine + O2 = dopaquinone + H2O | monophenol + O2 = o-diphenol + H2O, i.e. cresolase activity, proposed mechanism for sequence of hydroxylase and catecholase | Beta vulgaris |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.14.18.1 | (R)-dopaxanthin + dehydroascorbic acid + O2 | - |
Beta vulgaris | (R)-dopaxanthin quinone + L-ascorbic acid + H2O | - |
? | |
| 1.14.18.1 | caffeic acid + 1/2 O2 | - |
Beta vulgaris | caffeoyl quinone + H2O | - |
? | |
| 1.14.18.1 | p-coumaric acid + O2 + AH2 | artificial electron donors: NADH, dimethyltetrahydropteridine and ascorbic acid | Beta vulgaris | caffeic acid + H2O + A | - |
? | |
Ki Value [mM]
| EC Number | Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.14.18.1 | 0.0013 | - |
diethyldithiocarbamate | vs. O2 | Beta vulgaris | |
| 1.14.18.1 | 0.0119 | - |
diethyldithiocarbamate | vs. p-coumaric acid | Beta vulgaris | |
| 1.14.18.1 | 0.0162 | - |
CO | vs. O2 | Beta vulgaris | |
| 1.14.18.1 | 0.062 | - |
CO | vs. p-coumaric acid | Beta vulgaris | |
| 1.14.18.1 | 0.15 | - |
CO | vs. ascorbic acid | Beta vulgaris | |
| 1.14.18.1 | 0.277 | - |
Bathocuproinesulfonate | vs. caffeic acid | Beta vulgaris | |
| 1.14.18.1 | 0.284 | - |
Bathocuproinesulfonate | vs. O2 | Beta vulgaris | |
| 1.14.18.1 | 0.338 | - |
CO | vs. caffeic acid | Beta vulgaris | |
| 1.14.18.1 | 0.35 | - |
4-hydroxybenzoic acid | vs. O2 | Beta vulgaris | |
| 1.14.18.1 | 0.375 | - |
4-hydroxybenzoic acid | vs. ascorbic acid | Beta vulgaris | |
| 1.14.18.1 | 0.45 | - |
benzoic acid | vs. O2 | Beta vulgaris | |
| 1.14.18.1 | 0.5 | - |
4-hydroxybenzoic acid | vs. p-coumaric acid | Beta vulgaris | |
| 1.14.18.1 | 0.52 | - |
3,4-dihydroxybenzoic acid | vs. O2 | Beta vulgaris | |
| 1.14.18.1 | 0.561 | - |
Bathocuproinesulfonate | vs. p-coumaric acid | Beta vulgaris | |
| 1.14.18.1 | 0.7 | - |
benzoic acid | vs. p-coumaric acid | Beta vulgaris | |
| 1.14.18.1 | 0.75 | - |
benzoic acid | vs. caffeic acid | Beta vulgaris | |
| 1.14.18.1 | 0.75 | - |
4-hydroxybenzoic acid | vs. caffeic acid | Beta vulgaris | |
| 1.14.18.1 | 0.825 | - |
Bathocuproinesulfonate | vs. ascorbic acid | Beta vulgaris | |
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