Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Hearing, V.J.; Ekel, T.M.; Montague, P.M.; Hearing, E.D.; Nicholson, J.M.
    Mammalian tyrosinase: isolation by a simple new procedure and characterization of its steric requirements for cofactor activity (1978), Arch. Biochem. Biophys., 185, 407-418.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.14.18.1 L-Dopa and analogs, activation Mus musculus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.14.18.1 0.014
-
 N-acetyl-3,4-dihydroxyphenethylamine
-
 Mus musculus
1.14.18.1 0.016
-
 L-Dopa
-
 Mus musculus
1.14.18.1 0.02
-
 3,4-dihydroxyphenylethanol
-
 Mus musculus
1.14.18.1 0.022
-
 DL-dopa
-
 Mus musculus
1.14.18.1 0.024
-
 N-methyl-3,4-dihydroxyphenethylamine
-
 Mus musculus
1.14.18.1 0.026
-
 D-Dopa
-
 Mus musculus
1.14.18.1 0.046
-
 3,4-dihydroxyphenylalanine methyl ester
-
 Mus musculus
1.14.18.1 0.047
-
 3,4-Dihydroxyphenethylamine
-
 Mus musculus
1.14.18.1 0.094
-
 3,4,5-trihydroxyphenethylamine
-
 Mus musculus
1.14.18.1 0.102
-
 2,4,5-trihydroxyphenethylamine
-
 Mus musculus
1.14.18.1 0.145
-
 3,4-dihydroxyphenylserine
-
 Mus musculus
1.14.18.1 0.194
-
 3-(3,4-Dihydroxyphenyl)-2-methylalanine
-
 Mus musculus
1.14.18.1 0.23
-
 3,4-dihydroxyphenylglycol
-
 Mus musculus
1.14.18.1 0.305
-
 3,4,6-trihydroxyphenylalanine
-
 Mus musculus

Organism

EC Number Organism UniProt Comment Textmining
1.14.18.1 Mus musculus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.14.18.1 preparative PAGE Mus musculus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.14.18.1 melanoma cell
-
 Mus musculus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.14.18.1 0.032
-
-
 Mus musculus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.18.1 2,4,5-trihydroxyphenethylamine + O2
-
 Mus musculus ?
-
 ?
1.14.18.1 3,4,5-trihydroxyphenethylamine + O2
-
 Mus musculus ?
-
 r
1.14.18.1 3,4,6-trihydroxyphenylalanine + O2
-
 Mus musculus ?
-
 ?
1.14.18.1 3,4-dihydroxyphenethylamine + O2
-
 Mus musculus ?
-
 ?
1.14.18.1 3,4-dihydroxyphenylalanine methyl ester + O2
-
 Mus musculus ?
-
 ?
1.14.18.1 3,4-dihydroxyphenylethanol + O2
-
 Mus musculus 2-(3,4-dioxocyclohexa-1,5-dien-1-yl)ethanol + H2O
-
 r
1.14.18.1 3,4-dihydroxyphenylglycol + O2
-
 Mus musculus 2-(3,4-dioxocyclohexa-1,5-dien-1-yl)glycol + H2O
-
 r
1.14.18.1 3,4-dihydroxyphenylserine + O2
-
 Mus musculus ?
-
 ?
1.14.18.1 3-(3,4-dihydroxyphenyl)-2-methylalanine + O2
-
 Mus musculus ?
-
 ?
1.14.18.1 D-dopa + O2
-
 Mus musculus dopaquinone + H2O
-
 r
1.14.18.1 DL-DOPA + O2
-
 Mus musculus dopaquinone + H2O
-
 r
1.14.18.1 N-acetyl-3,4-dihydroxyphenethylamine + O2
-
 Mus musculus ?
-
 ?
1.14.18.1 N-methyl-3,4-dihydroxyphenethylamine + O2
-
 Mus musculus ?
-
 ?

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.14.18.1 0.0117
-
 tyrosine enzyme from malignant melanocytes Mus musculus
1.14.18.1 0.0217
-
 tyrosine enzyme from normal melanocytes Mus musculus