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Literature summary extracted from

  • Sharma, R.C.; Ali, R.
    Isolation and characterization of catechol oxidase from Solanum melongena (1980), Phytochemistry, 19, 1597-1600.
No PubMed abstract available

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.14.18.1 2,3-dimercapto-1-propanol 2 mM, 93% inhibition Solanum melongena
1.14.18.1 2-mercaptoethanol 2 mM, 93% inhibition Solanum melongena
1.14.18.1 L-ascorbic acid 2 mM, 78% inhibition Solanum melongena
1.14.18.1 L-cysteine 2 mM, 95% inhibition Solanum melongena

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.14.18.1 0.7
-
 catechol
-
 Solanum melongena
1.14.18.1 6.25
-
 Dopa
-
 Solanum melongena

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.14.18.1 particle-bound
-
 Solanum melongena
-
-
1.14.18.1 soluble
-
 Solanum melongena
-
-

Organism

EC Number Organism UniProt Comment Textmining
1.14.18.1 Solanum melongena
-
 eggplant
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.14.18.1 ammonium sulfate, Sephadex G-200 Solanum melongena

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.14.18.1 fruit
-
 Solanum melongena
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.14.18.1 336
-
-
 Solanum melongena

Storage Stability

EC Number Storage Stability Organism
1.14.18.1 0°C, presence of SDS protects against decomposition Solanum melongena

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.18.1 catechol + 1/2 O2
-
 Solanum melongena 1,2-benzoquinone + H2O
-
 ?
1.14.18.1 dopa + 1/2 O2
-
 Solanum melongena dopaquinone + H2O
-
 ?

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.14.18.1 6.5
-
 substrate catechol, 2 pH-maxima at 6.5 and 7.5 Solanum melongena
1.14.18.1 6.5
-
 substrate dopa, 2 pH-maxima at pH 6.5 and pH 7.9 Solanum melongena
1.14.18.1 7.5
-
 substrate catechol, 2 pH-maxima at 6.5 and 7.5 Solanum melongena
1.14.18.1 7.9
-
 substrate dopa, 2 pH-maxima at pH 6.5 and pH 7.9 Solanum melongena

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.14.18.1 5 9 pH 5.0: about 60% of maximal activity, pH 9.0: about 40% of maximal activity, dopa as substrate Solanum melongena
1.14.18.1 5 8 pH 5.0: about 55% of maximal activity, pH 9.0: about 15% of maximal activity, catechol as substrate Solanum melongena