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Literature summary extracted from

  • Tremolieres, M.; Bieth, J.G.
    Isolation and characterization of the polyphenoloxidase from senescent leaves of black poplar (1984), Phytochemistry, 23, 501-505.
No PubMed abstract available

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.14.18.1 ferulic acid activation Populus nigra
1.14.18.1 gallic acid activation Populus nigra
1.14.18.1 phosphate increase of activity Populus nigra

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.14.18.1 1,10-phenanthroline 1 mM, inactivation, half-life: 30 min Populus nigra
1.14.18.1 2,5-dihydroxybenzoic acid
-
 Populus nigra
1.14.18.1 2-hydroxybenzoic acid
-
 Populus nigra
1.14.18.1 3,4-dihydroxybenzoic acid
-
 Populus nigra
1.14.18.1 3-hydroxycinnamic acid
-
 Populus nigra
1.14.18.1 4-hydroxybenzoic acid
-
 Populus nigra
1.14.18.1 4-hydroxycinnamic acid
-
 Populus nigra
1.14.18.1 CN- 0.6 mM, 50% inhibition Populus nigra
1.14.18.1 Cu2+ inhibition at concentrations higher than 5 mM Populus nigra
1.14.18.1 ferulic acid
-
 Populus nigra
1.14.18.1 gallic acid
-
 Populus nigra
1.14.18.1 hydroquinone
-
 Populus nigra

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.14.18.1 5
-
 catechol
-
 Populus nigra
1.14.18.1 5
-
 4-methylcatechol
-
 Populus nigra

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.14.18.1 Co2+ 50 mM, 360% activation Populus nigra
1.14.18.1 copper activation at low concentrations, inhibition above 5 mM Populus nigra
1.14.18.1 Mn2+ activation Populus nigra
1.14.18.1 Ni2+ 50 mM, 20% activation Populus nigra

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.14.18.1 60000
-
 PAGE Populus nigra

Organism

EC Number Organism UniProt Comment Textmining
1.14.18.1 Populus nigra
-
 black poplar
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.14.18.1 ammonium sulfate, Sephadex G-75, DEAE-cellulose Populus nigra

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.14.18.1 leaf
-
 Populus nigra
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.18.1 2 hydroquinone + O2
-
 Populus nigra 2 quinone + 2 H2O
-
 ?
1.14.18.1 4-methylcatechol + O2
-
 Populus nigra 4-methyl-o-benzoquinone + H2O
-
 ?
1.14.18.1 caffeic acid + 1/2 O2
-
 Populus nigra caffeoyl quinone + H2O
-
 ?
1.14.18.1 catechol + 1/2 O2
-
 Populus nigra 1,2-benzoquinone + H2O
-
 ?
1.14.18.1 L-tyrosine + O2 + AH2 no activity with tyrosine Populus nigra L-dopa + H2O + A
-
 ?
1.14.18.1 p-coumaric acid + O2 + AH2
-
 Populus nigra caffeic acid + H2O + A
-
 ?

Subunits

EC Number Subunits Comment Organism
1.14.18.1 monomer 1 * 60000, SDS-PAGE Populus nigra

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.14.18.1 5.5
-
 phenol oxidase activity Populus nigra
1.14.18.1 7.5
-
 o-diphenolase Populus nigra

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
1.14.18.1 0.09
-
 hydroquinone
-
 Populus nigra
1.14.18.1 0.24
-
 4-hydroxycinnamic acid
-
 Populus nigra
1.14.18.1 0.35
-
 2,5-dihydroxybenzoic acid
-
 Populus nigra
1.14.18.1 1.75
-
 2-hydroxybenzoic acid
-
 Populus nigra
1.14.18.1 2
-
 4-hydroxybenzoic acid
-
 Populus nigra
1.14.18.1 2.5
-
 3-hydroxycinnamic acid
-
 Populus nigra
1.14.18.1 4.3
-
 3,4-dihydroxybenzoic acid
-
 Populus nigra