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Literature summary extracted from

  • Hsu, A.F.; Kalan, E.B.; Bills, D.D.
    Partial purification and characterization of the soluble polyphenol oxidases from suspension cultures of Papaver somniferum (1984), Plant Sci. Lett., 34, 315-322.
No PubMed abstract available

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.14.18.1 CN- 1 mM, 59% inhibition of isoenzyme a, 83% inhibition of isoenzyme B Papaver somniferum
1.14.18.1 diethyldithiocarbamate 1 mM, 34% inhibition of isoenzyme A, 95% inhibition of isoenzyme B Papaver somniferum
1.14.18.1 L-ascorbic acid
-
 Papaver somniferum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.14.18.1 5
-
 catechol
-
 Papaver somniferum
1.14.18.1 5
-
 catechol isoenzymes A and B Papaver somniferum

Organism

EC Number Organism UniProt Comment Textmining
1.14.18.1 Papaver somniferum
-
 opium, isoenzymes A and B
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.14.18.1 isoenzymes A and B, ammonium sulfate, CM-cellulose, Sephacryl S-200 Papaver somniferum

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.14.18.1 additional information
-
 isoenzyme A: 92.0 units, isoenzyme B: 97 units, 1 unit is defined as absorbance change of 0.001 at 430 nm per min Papaver somniferum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.18.1 catechol + 1/2 O2
-
 Papaver somniferum 1,2-benzoquinone + H2O
-
 ?
1.14.18.1 L-tyrosine + O2 + AH2 no activity with tyrosine Papaver somniferum L-dopa + H2O + A
-
 ?
1.14.18.1 p-cresol + O2 no activity Papaver somniferum 4-methylpyrocatechol + H2O
-
 ?