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Literature summary extracted from

  • Oda, Y.; Kato, H.; Isoda, Y.; Takahashi, N.; Yamamoto, T.; Takada, Y.; Kudo, S.
    Purification and properties of phenoloxidase from spinach leaves (1989), Agric. Biol. Chem., 53, 2053-2061.
No PubMed abstract available

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.14.18.1 Acetylacetone 0.1 mM, 75% inhibition Spinacia oleracea
1.14.18.1 Bathocuproine
-
 Spinacia oleracea
1.14.18.1 Cuprizone
-
 Spinacia oleracea
1.14.18.1 Cuproine
-
 Spinacia oleracea
1.14.18.1 diethyldithiocarbamate 0.1 mM, 80% inhibition Spinacia oleracea
1.14.18.1 DL-dithiothreitol
-
 Spinacia oleracea
1.14.18.1 EDTA no inhibition EDTA Spinacia oleracea
1.14.18.1 additional information not inhibited by 2,2'-dipyridyl Spinacia oleracea

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.14.18.1 2.87
-
 Protocatechuic aldehyde
-
 Spinacia oleracea
1.14.18.1 3.24
-
 esculetin
-
 Spinacia oleracea
1.14.18.1 4.75
-
 4-methylcatechol
-
 Spinacia oleracea
1.14.18.1 5.15
-
 chlorogenic acid
-
 Spinacia oleracea
1.14.18.1 6.43
-
 caffeic acid
-
 Spinacia oleracea
1.14.18.1 7.95
-
 catechol
-
 Spinacia oleracea
1.14.18.1 8.63
-
 Dopa
-
 Spinacia oleracea
1.14.18.1 27.4
-
 Hydrocaffeic acid
-
 Spinacia oleracea
1.14.18.1 189
-
 protocatechuic acid
-
 Spinacia oleracea

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.14.18.1 copper 2 atoms of copper per 150000 Da Spinacia oleracea

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.14.18.1 40000
-
 4 * 40000, SDS-PAGE Spinacia oleracea
1.14.18.1 150000
-
 gel filtration Spinacia oleracea

Organism

EC Number Organism UniProt Comment Textmining
1.14.18.1 Spinacia oleracea
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.14.18.1 salting out and Sephadex G-75, DEAE-cellulose, DEAE-Sephadex A-25, hydroxylapatite Spinacia oleracea

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.14.18.1 leaf
-
 Spinacia oleracea
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.14.18.1 0.277
-
 with catechol Spinacia oleracea

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.18.1 4-methylcatechol + O2
-
 Spinacia oleracea 4-methyl-o-benzoquinone + H2O
-
 ?
1.14.18.1 4-nitrocatechol + O2
-
 Spinacia oleracea ?
-
 ?
1.14.18.1 caffeic acid + 1/2 O2
-
 Spinacia oleracea caffeoyl quinone + H2O
-
 ?
1.14.18.1 catechol + 1/2 O2
-
 Spinacia oleracea 1,2-benzoquinone + H2O
-
 ?
1.14.18.1 chlorogenic acid + O2
-
 Spinacia oleracea chlorogenoquinone + H2O
-
 ?
1.14.18.1 dopa + 1/2 O2
-
 Spinacia oleracea dopaquinone + H2O
-
 ?
1.14.18.1 esculetin + O2
-
 Spinacia oleracea ?
-
 ?
1.14.18.1 hydrocaffeic acid + O2
-
 Spinacia oleracea ?
-
 ?
1.14.18.1 protocatechuic acid + O2
-
 Spinacia oleracea ?
-
 ?
1.14.18.1 protocatechuic aldehyde + O2
-
 Spinacia oleracea ?
-
 ?

Subunits

EC Number Subunits Comment Organism
1.14.18.1 tetramer 4 * 40000, SDS-PAGE Spinacia oleracea

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.14.18.1 123
-
 esculetin
-
 Spinacia oleracea
1.14.18.1 336
-
 Protocatechuic aldehyde
-
 Spinacia oleracea
1.14.18.1 860
-
 protocatechuic acid
-
 Spinacia oleracea
1.14.18.1 1450
-
 caffeic acid
-
 Spinacia oleracea
1.14.18.1 1650
-
 chlorogenic acid
-
 Spinacia oleracea
1.14.18.1 2960
-
 catechol
-
 Spinacia oleracea
1.14.18.1 4630
-
 Hydrocaffeic acid
-
 Spinacia oleracea
1.14.18.1 4940
-
 Dopa
-
 Spinacia oleracea
1.14.18.1 7220
-
 4-methylcatechol
-
 Spinacia oleracea

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.14.18.1 6.5
-
-
 Spinacia oleracea

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.14.18.1 5 8 high activity in the range pH 5-8 Spinacia oleracea