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Literature summary extracted from

  • Janovitz-Klapp, A.; Richard, F.; Nicholas, J.
    Polyphenoloxidase from apple, partial purification and some properties (1989), Phytochemistry, 28, 2903-2907.
No PubMed abstract available

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.14.18.1 4.1
-
 chlorogenic acid at pH 4.0, slight increase in Km above pH 5.0 Malus sp.
1.14.18.1 4.9
-
 chlorogenic acid at pH 5.5, slight increase in Km above pH 5.0 Malus sp.
1.14.18.1 5.2
-
 4-methylcatechol at pH 3.5-5.0, strong increase in Km above pH 5.0 Malus sp.
1.14.18.1 6.1
-
 (+)-catechin at pH 4.0, slight increase in Km above pH 4.0 Malus sp.
1.14.18.1 6.6
-
 (+)-catechin at pH 5.5, slight increase in Km above pH 4.0 Malus sp.
1.14.18.1 20.5
-
 4-methylcatechol at pH 6.3, strong increase in Km above pH 5.0 Malus sp.

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.14.18.1 46000
-
 gel filtration Malus sp.

Organism

EC Number Organism UniProt Comment Textmining
1.14.18.1 Malus sp.
-
 apple, var. red delicious
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.14.18.1 ammonium sulfate, Phenyl Sepharose Malus sp.

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.14.18.1 fruit peel and cortex Malus sp.
-
1.14.18.1 peel
-
 Malus sp.
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.14.18.1 11330
-
-
 Malus sp.

Storage Stability

EC Number Storage Stability Organism
1.14.18.1 4°C, 15 days, 10% loss of activity Malus sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.18.1 4-methylcatechol + O2
-
 Malus sp. 4-methyl-o-benzoquinone + H2O
-
 ?
1.14.18.1 chlorogenic acid + O2
-
 Malus sp. chlorogenoquinone + H2O
-
 ?
1.14.18.1 D-catechin + O2
-
 Malus sp. ?
-
 ?

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.14.18.1 30
-
 4 h, pH 4.5, 50% loss of activity Malus sp.

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.14.18.1 4.5 5 with 4-methylcatechol, chlorogenic acid and (+)-catechin Malus sp.