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Literature summary extracted from

  • Nishikimi, M.; Tolbert, B.M.; Udenfriend, S.
    Purification and characterization of L-gulono-gamma-lactone oxidase from rat and goat liver (1976), Arch. Biochem. Biophys., 175, 427-435.
    View publication on PubMed

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.3.8 0.066
-
 L-gulono-1,4-lactone
-
 Rattus norvegicus
1.1.3.8 0.15
-
 L-gulono-1,4-lactone
-
 Capra hircus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.3.8 51000
-
 x * 51000, SDS-PAGE Rattus norvegicus
1.1.3.8 51000
-
 x * 51000, SDS-PAGE Capra hircus
1.1.3.8 500000
-
 gel filtration Rattus norvegicus
1.1.3.8 500000
-
 gel filtration Capra hircus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.3.8 L-gulono-1,4-lactone + O2 Rattus norvegicus
-
 L-xylo-hex-3-ulonolactone + H2O2
-
 ?
1.1.3.8 L-gulono-1,4-lactone + O2 Capra hircus
-
 L-xylo-hex-3-ulonolactone + H2O2
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.1.3.8 Capra hircus
-
-
-
1.1.3.8 Rattus norvegicus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.3.8
-
 Rattus norvegicus
1.1.3.8
-
 Capra hircus

Reaction

EC Number Reaction Comment Organism Reaction ID
1.1.3.8 L-gulono-1,4-lactone + O2 = L-xylo-hex-2-ulono-1,4-lactone + H2O2 product isomerizes spontaneously to L-ascorbate Rattus norvegicus
a
1.1.3.8 L-gulono-1,4-lactone + O2 = L-xylo-hex-2-ulono-1,4-lactone + H2O2 product isomerizes spontaneously to L-ascorbate Capra hircus
a

Source Tissue

EC Number Source Tissue Comment Organism Textmining

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.3.8 0.54
-
-
 Rattus norvegicus
1.1.3.8 2.05
-
-
 Capra hircus

Storage Stability

EC Number Storage Stability Organism
1.1.3.8 -20 C, 1 month, 100% activity Rattus norvegicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.3.8 L-gulono-1,4-lactone + O2
-
 Rattus norvegicus L-xylo-hex-3-ulonolactone + H2O2
-
 ?
1.1.3.8 L-gulono-1,4-lactone + O2
-
 Capra hircus L-xylo-hex-3-ulonolactone + H2O2
-
 ?
1.1.3.8 L-gulono-1,4-lactone + O2
-
 Rattus norvegicus L-xylo-hex-3-ulonolactone + H2O2 product isomerizes spontaneously to L-ascorbate ?

Subunits

EC Number Subunits Comment Organism
1.1.3.8 oligomer x * 51000, SDS-PAGE Rattus norvegicus
1.1.3.8 oligomer x * 51000, SDS-PAGE Capra hircus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.1.3.8 37
-
-
 Rattus norvegicus
1.1.3.8 37
-
-
 Capra hircus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.3.8 7.5
-
-
 Rattus norvegicus
1.1.3.8 7.5
-
-
 Capra hircus

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.3.8 flavin covalently bound, flavoenzyme Rattus norvegicus
1.1.3.8 flavin covalently bound, flavoenzyme Capra hircus