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Literature summary extracted from

  • Krasnov, A.; Reinisalo, M.; Pitk§nen, T.I.; Nishikimi, M.; M¸lsa, H.
    Expression of rat gene L-gulono-gamma-lactone oxidase, the key enzyme of L-ascorbic acid biosynthesis, in guinea pig cells and in teleost fish rainbow trout (Oncorhynchus mykiss) (1998), Biochim. Biophys. Acta, 1381, 241-248.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.3.8 expressed in Escherichia coli JM109, transfected into guinea pig cell line 104C1 Rattus norvegicus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.1.3.8 microsome integral membrane protein in the endoplasmic reticulum Rattus norvegicus
-
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.3.8 L-gulono-1,4-lactone + O2 Rattus norvegicus
-
 L-xylo-hex-3-ulonolactone + H2O2
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.1.3.8 no activity in Cavia porcellus
-
 guinea pig
-
1.1.3.8 no activity in Oncorhynchus mykiss
-
 teleost fish rainbow trout
-
1.1.3.8 Rattus norvegicus
-
-
-

Reaction

EC Number Reaction Comment Organism Reaction ID
1.1.3.8 L-gulono-1,4-lactone + O2 = L-xylo-hex-2-ulono-1,4-lactone + H2O2 product isomerizes spontaneously to L-ascorbate Rattus norvegicus
a

Source Tissue

EC Number Source Tissue Comment Organism Textmining

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.3.8 100
-
 after transfection in guinea pig cells Rattus norvegicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.3.8 L-gulono-1,4-lactone + O2
-
 Rattus norvegicus L-xylo-hex-3-ulonolactone + H2O2
-
 ?
1.1.3.8 L-gulono-1,4-lactone + O2
-
 Rattus norvegicus L-xylo-hex-3-ulonolactone + H2O2 product isomerizes spontaneously to L-ascorbate ?

Synonyms

EC Number Synonyms Comment Organism
1.1.3.8 GLO
-
 Rattus norvegicus

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.3.8 flavin covalently bound, flavoenzyme Rattus norvegicus