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Literature summary extracted from

  • Leichus, B.N.; Blanchard, J.S.
    Isotopic analysis of the reaction catalyzed by glycerol dehydrogenase (1994), Biochemistry, 33, 14642-14649.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.1.6 1,3-Propanediol competitive inhibitor versus glycerol in direction of glycerol oxidation, noncompetitive inhibitor versus NAD+, competitive inhibitor versus dihydroxyacetone in direction of glycerol reduction, noncompetitive inhibitor versus NADH Cellulomonas sp.
1.1.1.6 NADH competitive inhibitor versus NAD+ in direction of glycerol oxidation, noncompetitive inhibitor versus glycerol Cellulomonas sp.

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.6 0.06
-
 1,2-propanediol pH 8.8, 25°C Cellulomonas sp.
1.1.1.6 4
-
 3-mercapto-1,2-propanediol pH 8.8, 25°C Cellulomonas sp.
1.1.1.6 4.4
-
 R-1-amino-2-propanol pH 8.8, 25°C Cellulomonas sp.
1.1.1.6 5.1
-
 glycerol pH 8.8, 25°C Cellulomonas sp.
1.1.1.6 6
-
 3-Chloro-1,2-propanediol pH 8.8, 25°C Cellulomonas sp.
1.1.1.6 6.1
-
 3-bromo-1,2-propanediol pH 8.8, 25°C Cellulomonas sp.
1.1.1.6 180
-
 3-amino-1,2-propanediol pH 8.8, 25°C Cellulomonas sp.
1.1.1.6 500
-
 S-1-amino-2-propanol pH 8.8, 25°C Cellulomonas sp.

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.1.1.6 cytoplasm soluble, isolation from crude cell extract Cellulomonas sp. 5737
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.1.1.6 K+ required for enzymatic activity and glycerol binding Cellulomonas sp.

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.6 39500
-
 8 * 39500, SDS-PAGE Cellulomonas sp.
1.1.1.6 324000
-
 gel filtration Cellulomonas sp.

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.6 glycerol + NAD+ Cellulomonas sp.
-
 dihydroxyacetone + NADH + H+
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.6 Cellulomonas sp.
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.6
-
 Cellulomonas sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.6 1,2-propanediol + NAD+ 105% of the activity with glycerol Cellulomonas sp. hydroxyacetone + NADH
-
 ?
1.1.1.6 3-amino-1,2-propanediol + NAD+ 104% of the activity with glycerol Cellulomonas sp. ?
-
 ?
1.1.1.6 3-bromo-1,2-propanediol + NAD+ 109% of the activity with glycerol Cellulomonas sp. ?
-
 ?
1.1.1.6 3-chloro-1,2-propanediol + NAD+ 130% of the activity with glycerol Cellulomonas sp. ?
-
 ?
1.1.1.6 3-mercapto-1,2-propanediol + NAD+ 155% of the activity with glycerol Cellulomonas sp. ?
-
 ?
1.1.1.6 glycerol + NAD+
-
 Cellulomonas sp. dihydroxyacetone + NADH + H+
-
 ?
1.1.1.6 glycerol + NAD+
-
 Cellulomonas sp. dihydroxyacetone + NADH + H+
-
 r
1.1.1.6 R-1-amino-2-propanol + NAD+ 33% of the activity with glycerol Cellulomonas sp. ?
-
 ?
1.1.1.6 S-1-amino-2-propanol + NAD+ 9% of the activity with glycerol Cellulomonas sp. ?
-
 ?

Subunits

EC Number Subunits Comment Organism
1.1.1.6 octamer 8 * 39500, SDS-PAGE Cellulomonas sp.

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.6 NAD+
-
 Cellulomonas sp.
1.1.1.6 NADH
-
 Cellulomonas sp.