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Literature summary extracted from

  • Nishise, H.; Nagao, A.; Tani, Y.; Yamada, H.
    Further characterization of glycerol dehydrogenase from Cellulomonas sp. NT3060 (1984), Agric. Biol. Chem., 48, 1603-1609.
No PubMed abstract available

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.1.1.6 NH4+
-
 Cellulomonas sp.

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
1.1.1.6
-
 Cellulomonas sp.

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.1.6 dihydroxyacetone noncompatible inhibitor, at saturated level of glycerol Cellulomonas sp.
1.1.1.6 Hydroxyacetone
-
 Cellulomonas sp.
1.1.1.6 NADH
-
 Cellulomonas sp.

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.6 0.385
-
 dihydroxyacetone
-
 Cellulomonas sp.
1.1.1.6 10.9
-
 glycerol
-
 Cellulomonas sp.

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.1.1.6 cytoplasm soluble, isolation from crude cell extract Cellulomonas sp. 5737
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.1.1.6 K+ oxidation of glycerol os greatly increased Cellulomonas sp.

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.6 336000 390000 gel filtration Cellulomonas sp.
1.1.1.6 336000 390000 sedimentation equilibrium method Cellulomonas sp.

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.6 glycerol + NAD+ Cellulomonas sp.
-
 dihydroxyacetone + NADH + H+
-
 ?
1.1.1.6 glycerol + NAD+ Cellulomonas sp. NT3060
-
 dihydroxyacetone + NADH + H+
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.6 Cellulomonas sp.
-
-
-
1.1.1.6 Cellulomonas sp. NT3060
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.6
-
 Cellulomonas sp.

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.1.6 128
-
-
 Cellulomonas sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.6 1,2-propanediol + NAD+
-
 Cellulomonas sp. hydroxyacetone + NADH
-
 r
1.1.1.6 1,2-propanediol + NAD+
-
 Cellulomonas sp. NT3060 hydroxyacetone + NADH
-
 r
1.1.1.6 2,3-butanediol + NAD+
-
 Cellulomonas sp. 3-hydroxybutane-2-one + NADH
-
 r
1.1.1.6 2,3-butanediol + NAD+
-
 Cellulomonas sp. NT3060 3-hydroxybutane-2-one + NADH
-
 r
1.1.1.6 glycerol + NAD+
-
 Cellulomonas sp. dihydroxyacetone + NADH + H+
-
 ?
1.1.1.6 glycerol + NAD+
-
 Cellulomonas sp. NT3060 dihydroxyacetone + NADH + H+
-
 ?

Subunits

EC Number Subunits Comment Organism
1.1.1.6 octamer 8 * 42000-43000, SDS-PAGE Cellulomonas sp.

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.1.1.6 50
-
 oxidation of glycerol Cellulomonas sp.
1.1.1.6 50
-
 oxidation of butanediol Cellulomonas sp.
1.1.1.6 65
-
 oxidation of propanediol Cellulomonas sp.

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.6 5
-
 reduction of hydroxyacetone Cellulomonas sp.
1.1.1.6 6
-
 reduction of dihydroxyacetone or 3-hydroxybutane-2-one Cellulomonas sp.
1.1.1.6 8 8.5 oxidation of 1,2-propanediol Cellulomonas sp.
1.1.1.6 9
-
 oxidation of glycerol or butanediol Cellulomonas sp.

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.6 NAD+
-
 Cellulomonas sp.
1.1.1.6 NADH
-
 Cellulomonas sp.

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
1.1.1.6 11.1
-
 dihydroxyacetone
-
 Cellulomonas sp.
1.1.1.6 11.6
-
 Hydroxyacetone
-
 Cellulomonas sp.