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Literature summary extracted from

  • Gärtner, G.; Kopperschläger, G.
    Purification and properties of glycerol dehydrogenase from Candida valida (1984), J. Gen. Microbiol., 130, 3225-3233.
No PubMed abstract available

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.1.1.6 citrate the enzyme is more active in presence of multivalent anions, citrate, phosphate or sulfate, in comparison to monovalent anions, e.g. acetate or chloride Pichia membranifaciens
1.1.1.6 phosphate the enzyme is more active in presence of multivalent anions, citrate, phosphate or sulfate, in comparison to monovalent anions, e.g. acetate or chloride Pichia membranifaciens
1.1.1.6 SO42- the enzyme is more active in presence of multivalent anions, citrate, phosphate or sulfate, in comparison to monovalent anions, e.g. acetate or chloride Pichia membranifaciens

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.1.6 2-amino-2-(hydroxymethyl)-1,3-propanediol strong inhibition, IC50 2 mM Pichia membranifaciens
1.1.1.6 2-mercaptoethanol strong inhibition, IC50 0.3 mM Pichia membranifaciens
1.1.1.6 dihydroxyacetone phosphate
-
 Pichia membranifaciens
1.1.1.6 dithiothreitol slight inhibition Pichia membranifaciens
1.1.1.6 ethylene glycol strong inhibition, IC50 4 mM Pichia membranifaciens
1.1.1.6 glycerol 3-phosphate
-
 Pichia membranifaciens
1.1.1.6 L-cysteine slight inhibition Pichia membranifaciens
1.1.1.6 N-ethylmaleimide rapid inactivation, IC50: 0.2 mM Pichia membranifaciens
1.1.1.6 NAD+
-
 Pichia membranifaciens
1.1.1.6 NADH product inhibition , competitive with NAD+ Pichia membranifaciens
1.1.1.6 NADP+
-
 Pichia membranifaciens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.6 0.048
-
 NADH 25°C Pichia membranifaciens
1.1.1.6 0.14
-
 NAD+ 25°C Pichia membranifaciens
1.1.1.6 0.77
-
 dihydroxyacetone 25°C Pichia membranifaciens
1.1.1.6 58
-
 glycerol 25°C Pichia membranifaciens

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.1.1.6 cytoplasm soluble, isolation from crude cell extract Pichia membranifaciens 5737
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.6 38000
-
 2 * 38000, SDS-PAGE Pichia membranifaciens
1.1.1.6 76000 80000 gel filtration, PAGE under nondenaturing conditions Pichia membranifaciens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.6 glycerol + NAD+ Pichia membranifaciens
-
 dihydroxyacetone + NADH + H+
-
 ?
1.1.1.6 glycerol + NAD+ Pichia membranifaciens H 112
-
 dihydroxyacetone + NADH + H+
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.6 Pichia membranifaciens
-
-
-
1.1.1.6 Pichia membranifaciens H 112
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.6
-
 Pichia membranifaciens

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.1.6 448
-
-
 Pichia membranifaciens

Storage Stability

EC Number Storage Stability Organism
1.1.1.6 -15°C, with 5% culture filtrate or 5% boiled crude cell extract, 10% Ficoll, pH 7.5, stable for 1 month Pichia membranifaciens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.6 1,2-propanediol + NAD+
-
 Pichia membranifaciens hydroxyacetone + NADH
-
 ?
1.1.1.6 1,2-propanediol + NAD+
-
 Pichia membranifaciens H 112 hydroxyacetone + NADH
-
 ?
1.1.1.6 DL-glyceraldehyde + NAD+
-
 Pichia membranifaciens 3-hydroxypyruvaldehyde + NADH
-
 ?
1.1.1.6 DL-glyceraldehyde + NAD+
-
 Pichia membranifaciens H 112 3-hydroxypyruvaldehyde + NADH
-
 ?
1.1.1.6 glycerol + NAD+
-
 Pichia membranifaciens dihydroxyacetone + NADH + H+
-
 ?
1.1.1.6 glycerol + NAD+
-
 Pichia membranifaciens H 112 dihydroxyacetone + NADH + H+
-
 ?

Subunits

EC Number Subunits Comment Organism
1.1.1.6 dimer 2 * 38000, SDS-PAGE Pichia membranifaciens

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.1.1.6 36
-
-
 Pichia membranifaciens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.6 7.5 8.5 reduction of dihydroxyacetone, phosphate buffer Pichia membranifaciens
1.1.1.6 8
-
 oxidation of glycerol, tetraethyl ammonium chloride/tetraethylammonium hydroxide buffer Pichia membranifaciens
1.1.1.6 9.5 10.5 oxidation of glycerol, diphosphate buffer Pichia membranifaciens

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
1.1.1.6 7.5
-
 most stable Pichia membranifaciens

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.6 NAD+
-
 Pichia membranifaciens
1.1.1.6 NADH
-
 Pichia membranifaciens

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
1.1.1.6 0.025
-
 NADH 25°C Pichia membranifaciens
1.1.1.6 1.09
-
 NAD+ 25°C Pichia membranifaciens

IC50 Value

EC Number IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
1.1.1.6 0.2
-
 rapid inactivation, IC50: 0.2 mM Pichia membranifaciens N-ethylmaleimide
1.1.1.6 0.3
-
 strong inhibition, IC50 0.3 mM Pichia membranifaciens 2-mercaptoethanol
1.1.1.6 2
-
 strong inhibition, IC50 2 mM Pichia membranifaciens 2-amino-2-(hydroxymethyl)-1,3-propanediol
1.1.1.6 4
-
 strong inhibition, IC50 4 mM Pichia membranifaciens ethylene glycol