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Literature summary extracted from

  • Meyer, M.; Granderath, K.; Andreesen, J.R.
    Purification and characterization of protein PB of betaine reductase and its relationship to the corresponding proteins glycine reductase and sarcosine reductase from Eubacterium acidaminophilum (1995), Eur. J. Biochem., 234, 184-191.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.21.4.3 hydroxylamine
-
 Peptoclostridium acidaminophilum

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.21.4.4 45000
-
 alpha2,beta2, 2 * 45000 + 2 * 48000, SDS-PAGE Peptoclostridium acidaminophilum
1.21.4.4 48000
-
 alpha2,beta2, 2 * 45000 + 2 * 48000, SDS-PAGE Peptoclostridium acidaminophilum
1.21.4.4 200000
-
 gel filtration Peptoclostridium acidaminophilum

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.21.4.3 N-methylglycine + phosphate + thioredoxin Peptoclostridium acidaminophilum
-
 acetyl phosphate + methylamine + thioredoxin disulfide
-
 ?
1.21.4.3 N-methylglycine + phosphate + thioredoxin Peptoclostridium litorale
-
 acetyl phosphate + methylamine + thioredoxin disulfide
-
 ?
1.21.4.4 N,N,N-trimethylglycine + phosphate + thioredoxin Peptoclostridium acidaminophilum
-
 acetyl phosphate + trimethylamine + thioredoxin disulfide
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.21.4.3 Peptoclostridium acidaminophilum
-
 DSM 3953
-
1.21.4.3 Peptoclostridium litorale
-
 DSM 5388, expression of enzyme is induced only when grown on sarcosine
-
1.21.4.4 Peptoclostridium acidaminophilum
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.21.4.4 homogeneity, betaine specific subunits of protein B Peptoclostridium acidaminophilum

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.21.4.3 114
-
 pH 7, 37°C, extract from bacteria that were grown on alanine and sarcosine Peptoclostridium litorale
1.21.4.3 229
-
 pH 7, 37°C, extract from bacteria that were grown on formate and sarcosine Peptoclostridium acidaminophilum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.21.4.3 N-methylglycine + phosphate + thioredoxin
-
 Peptoclostridium acidaminophilum acetyl phosphate + methylamine + thioredoxin disulfide
-
 ?
1.21.4.3 N-methylglycine + phosphate + thioredoxin
-
 Peptoclostridium litorale acetyl phosphate + methylamine + thioredoxin disulfide
-
 ?
1.21.4.4 N,N,N-trimethylglycine + phosphate + thioredoxin
-
 Peptoclostridium acidaminophilum acetyl phosphate + trimethylamine + thioredoxin disulfide
-
 ?

Subunits

EC Number Subunits Comment Organism
1.21.4.4 tetramer alpha2,beta2, 2 * 45000 + 2 * 48000, SDS-PAGE Peptoclostridium acidaminophilum

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.21.4.4 37
-
 assay at Peptoclostridium acidaminophilum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.21.4.3 7
-
-
 Peptoclostridium acidaminophilum