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Literature summary extracted from

  • Ploux, O.; Soularue, P.; Marquet, A.; Gloeckler, R.; Lemoine, Y.
    Investigation of the first step of biotin biosynthesis in Bacillus sphaericus. Purification and characterization of the pimeloyl-CoA synthetase, and uptake of pimelate (1992), Biochem. J., 287, 685-690.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
6.2.1.14 overexpression in Escherichia coli Lysinibacillus sphaericus

Inhibitors

EC Number Inhibitors Comment Organism Structure
6.2.1.14 1,10-phenanthroline
-
 Lysinibacillus sphaericus
6.2.1.14 EDTA
-
 Lysinibacillus sphaericus
6.2.1.14 Mg2+ inhibition above 10 mM, requirement at lower concentrations Lysinibacillus sphaericus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
6.2.1.14 0.033
-
 CoA
-
 Lysinibacillus sphaericus
6.2.1.14 0.145
-
 heptanedioate
-
 Lysinibacillus sphaericus
6.2.1.14 0.17
-
 MgATP2-
-
 Lysinibacillus sphaericus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
6.2.1.14 Co2+ 32% of the activation relative to Mg2+ Lysinibacillus sphaericus
6.2.1.14 Mg2+ required Lysinibacillus sphaericus
6.2.1.14 Mg2+ Km: 2.3 mM Lysinibacillus sphaericus
6.2.1.14 Mg2+ inhibition above 10 mM Lysinibacillus sphaericus
6.2.1.14 Mn2+ 73% of the activation relative to Mg2+ Lysinibacillus sphaericus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
6.2.1.14 28000
-
 2 * 28000, SDS-PAGE Lysinibacillus sphaericus
6.2.1.14 60000
-
 gel filtration Lysinibacillus sphaericus

Organism

EC Number Organism UniProt Comment Textmining
6.2.1.14 Lysinibacillus sphaericus
-
-
-
6.2.1.14 Lysinibacillus sphaericus
-
 expressed in an overproducing Escherichia coli strain C268
-
6.2.1.14 Lysinibacillus sphaericus IFO 3525
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
6.2.1.14
-
 Lysinibacillus sphaericus

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
6.2.1.14 1
-
-
 Lysinibacillus sphaericus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.2.1.14 ATP + 6-carboxyhexanoate + CoA
-
 Lysinibacillus sphaericus AMP + diphosphate + 6-carboxyhexanoyl-CoA
-
 ?
6.2.1.14 ATP + 6-carboxyhexanoate + CoA
-
 Lysinibacillus sphaericus IFO 3525 AMP + diphosphate + 6-carboxyhexanoyl-CoA
-
 ?
6.2.1.14 ATP + heptanedioate + CoA
-
 Lysinibacillus sphaericus AMP + diphosphate + heptanedioyl-CoA heptanedioyl-CoA i.e. pimelyl-CoA ?
6.2.1.14 ATP + heptanedioate + CoA
-
 Lysinibacillus sphaericus IFO 3525 AMP + diphosphate + heptanedioyl-CoA heptanedioyl-CoA i.e. pimelyl-CoA ?

Subunits

EC Number Subunits Comment Organism
6.2.1.14 dimer 2 * 28000, SDS-PAGE Lysinibacillus sphaericus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
6.2.1.14 additional information
-
 additional information
-
 Lysinibacillus sphaericus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
6.2.1.14 8.5 9
-
 Lysinibacillus sphaericus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
6.2.1.14 8 9.5 8: about 55% of maximal activity, 9.5: about 65% of maximal activity Lysinibacillus sphaericus