EC Number | General Stability | Organism |
---|---|---|
6.2.1.13 | salts stabilize against heat inactivation. In presence of 1 M KCl the enzyme does not lose activity after 2 h incubation | Pyrococcus furiosus |
6.2.1.13 | sensitivity towards heat inactivation is increased with storage at -20°C | Pyrococcus furiosus |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
6.2.1.13 | 0.017 | - |
acetyl-CoA | - |
Pyrococcus furiosus | |
6.2.1.13 | 0.03 | - |
CoA | - |
Pyrococcus furiosus | |
6.2.1.13 | 0.06 | - |
ADP | - |
Pyrococcus furiosus | |
6.2.1.13 | 0.08 | - |
ADP | - |
Pyrococcus furiosus | |
6.2.1.13 | 0.2 | - |
phosphate | - |
Pyrococcus furiosus | |
6.2.1.13 | 0.66 | - |
acetate | - |
Pyrococcus furiosus |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
6.2.1.13 | Ca2+ | no effect | Pyrococcus furiosus | |
6.2.1.13 | Co2+ | can replace Mg2+ in activation | Pyrococcus furiosus | |
6.2.1.13 | Co2+ | 44% of the activation relative to Mg2+ | Pyrococcus furiosus | |
6.2.1.13 | Mg2+ | required | Pyrococcus furiosus | |
6.2.1.13 | Mg2+ | Km: 0.2 mM | Pyrococcus furiosus | |
6.2.1.13 | Mg2+ | an Mg2+/ATP ratio of up to 10 is required for optimal activity, suggesting that Mg2+ not only complexes ADP but has an additional stimulating effect on enzyme function | Pyrococcus furiosus | |
6.2.1.13 | Mn2+ | can replace Mg2+ in activation | Pyrococcus furiosus | |
6.2.1.13 | Mn2+ | 68% of the activation relative to Mg2+ | Pyrococcus furiosus |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
6.2.1.13 | 25000 | - |
2 * 47000 (alpha) + 2 * 25000 (beta), SDS-PAGE | Pyrococcus furiosus |
6.2.1.13 | 47000 | - |
2 * 47000 (alpha) + 2 * 25000 (beta), SDS-PAGE | Pyrococcus furiosus |
6.2.1.13 | 145000 | - |
gel filtration | Pyrococcus furiosus |
6.2.1.13 | 150000 | 165000 | native PAGE | Pyrococcus furiosus |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
6.2.1.13 | ADP + phosphate + acetyl-CoA | Pyrococcus furiosus | - |
? | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
6.2.1.13 | Pyrococcus furiosus | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
6.2.1.13 | - |
Pyrococcus furiosus |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
6.2.1.13 | additional information | - |
- |
Pyrococcus furiosus |
EC Number | Storage Stability | Organism |
---|---|---|
6.2.1.13 | -20°C, 1-2 mg/ml enzyme, in 20 mM Tris/HCl, pH 8.0, 2 mM MgCl2, 150 mM NaCl, stable for several weeks | Pyrococcus furiosus |
6.2.1.13 | on ice stable for 2 d | Pyrococcus furiosus |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
6.2.1.13 | ADP + phosphate + acetyl-CoA | r | Pyrococcus furiosus | ATP + acetate + CoA | - |
? | |
6.2.1.13 | ADP + phosphate + acetyl-CoA | - |
Pyrococcus furiosus | ? | - |
? | |
6.2.1.13 | ADP + phosphate + butyryl-CoA | 92% of the activity relative to acetyl-CoA | Pyrococcus furiosus | ATP + butyrate + CoA | - |
? | |
6.2.1.13 | ADP + phosphate + propionyl-CoA | r | Pyrococcus furiosus | ATP + propionate + CoA | - |
? | |
6.2.1.13 | ADP + phosphate + propionyl-CoA | 110% of the activity relative to acetyl-CoA | Pyrococcus furiosus | ATP + propionate + CoA | - |
? | |
6.2.1.13 | ATP + isobutyrate + CoA | 79% of the activity relative to acetate | Pyrococcus furiosus | ADP + phosphate + isobutyryl-CoA | - |
? | |
6.2.1.13 | ATP + isopentanioate + CoA | 34% of the activity relative to acetate | Pyrococcus furiosus | ADP + phosphate + isovaleryl-CoA | - |
? | |
6.2.1.13 | ATP + pentanoate + CoA | 36% of the activity relative to acetate | Pyrococcus furiosus | ADP + phosphate + valeryl-CoA | - |
? | |
6.2.1.13 | GDP + phosphate + acetyl-CoA | r, 220% of the activity relative to ADP | Pyrococcus furiosus | GTP + acetate + CoA | - |
? | |
6.2.1.13 | IDP + phosphate + acetyl-CoA | r, 250% of the activity relative to ADP | Pyrococcus furiosus | ITP + acetate + CoA | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
6.2.1.13 | tetramer | 2 * 47000 (alpha) + 2 * 25000 (beta), SDS-PAGE | Pyrococcus furiosus |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
6.2.1.13 | 90 | - |
- |
Pyrococcus furiosus |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
6.2.1.13 | 100 | - |
half-life is 60 min, in presence of 1 M KCl no loss of activity after 2 h | Pyrococcus furiosus |
6.2.1.13 | 110 | - |
half-life is 30 min | Pyrococcus furiosus |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
6.2.1.13 | 7 | - |
- |
Pyrococcus furiosus |
EC Number | pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|---|
6.2.1.13 | 6 | 8 | about 50% of maximal activity at pH 6 and 8 | Pyrococcus furiosus |