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Literature summary extracted from

  • Sanchez, L.B.; Muller, M.
    Purification and characterization of the acetate forming enzyme, acetyl-CoA synthetase (ADP-forming) from the amitochondriate protist, Giardia lamblia (1996), FEBS Lett., 378, 240-244.
    View publication on PubMed

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
6.2.1.13 0.02
-
 CoA acetyl-CoA Giardia intestinalis
6.2.1.13 0.23
-
 ADP
-
 Giardia intestinalis
6.2.1.13 1.09
-
 dADP
-
 Giardia intestinalis

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
6.2.1.13 Ca2+ can replace Mg2+ in activition, with 15% of the efficiency Giardia intestinalis
6.2.1.13 Mg2+ required Giardia intestinalis
6.2.1.13 Mg2+ Km: 0.59 Giardia intestinalis
6.2.1.13 Mn2+ can replace Mg2+ in activation Giardia intestinalis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
6.2.1.13 ADP + phosphate + acetyl-CoA Giardia intestinalis
-
 ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
6.2.1.13 Giardia intestinalis
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
6.2.1.13
-
 Giardia intestinalis

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
6.2.1.13 178
-
-
 Giardia intestinalis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.2.1.13 ADP + phosphate + acetyl-CoA reverse reaction not detected Giardia intestinalis ATP + acetate + CoA
-
 ?
6.2.1.13 ADP + phosphate + acetyl-CoA
-
 Giardia intestinalis ?
-
 ?
6.2.1.13 ADP + phosphate + butyryl-CoA no activity with n-butyryl-CoA Giardia intestinalis ATP + butyrate + CoA
-
 ?
6.2.1.13 ADP + phosphate + propionyl-CoA 39% of the activity relative to acetyl-CoA Giardia intestinalis ATP + propionate + CoA
-
 ?
6.2.1.13 ADP + phosphate + succinyl-CoA 30% of the activity relative to acetyl-CoA Giardia intestinalis ATP + succinate + CoA
-
 ?
6.2.1.13 dADP + phosphate + acetyl-CoA 40% of the activity relative to ADP Giardia intestinalis dATP + acetate + CoA
-
 ?

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
6.2.1.13 7.2
-
-
 Giardia intestinalis