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Literature summary extracted from

  • Feutry, A.; Letouze, R.
    Properties of hydroxycinnamate:CoA ligase from stems of Salix babylonica cultured in vitro (1984), Phytochemistry, 23, 1557-1559.
No PubMed abstract available

Inhibitors

EC Number Inhibitors Comment Organism Structure
6.2.1.12 4-coumarate inhibition above 0.6 mM Salix babylonica
6.2.1.12 caffeate inhibition above 0.2 mM Salix babylonica
6.2.1.12 ferulate inhibition above 0.4 mM Salix babylonica

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
6.2.1.12 0.0047
-
ferulate
-
Salix babylonica
6.2.1.12 0.031
-
4-coumarate
-
Salix babylonica
6.2.1.12 0.032
-
4-coumarate ATP Salix babylonica
6.2.1.12 0.046
-
ferulate
-
Salix babylonica

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
6.2.1.12 Mg2+ required Salix babylonica
6.2.1.12 Mg2+ optimal concentration 10 mM Salix babylonica

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
6.2.1.12 57000
-
gel filtration Salix babylonica

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
6.2.1.12 ATP + 4-coumarate + CoA Salix babylonica physiological role in lignin biosynthesis ?
-
?

Organism

EC Number Organism UniProt Comment Textmining
6.2.1.12 Salix babylonica
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
6.2.1.12
-
Salix babylonica

Source Tissue

EC Number Source Tissue Comment Organism Textmining
6.2.1.12 stem
-
Salix babylonica
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.2.1.12 ATP + 4-coumarate + CoA
-
Salix babylonica AMP + diphosphate + 4-coumaroyl-CoA
-
?
6.2.1.12 ATP + 4-coumarate + CoA physiological role in lignin biosynthesis Salix babylonica ?
-
?
6.2.1.12 ATP + caffeate + CoA
-
Salix babylonica AMP + diphosphate + caffeoyl-CoA
-
?
6.2.1.12 ATP + ferulate + CoA
-
Salix babylonica AMP + diphosphate + feruloyl-CoA
-
?

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
6.2.1.12 7
-
-
Salix babylonica

pH Range

EC Number pH Minimum pH Maximum Comment Organism
6.2.1.12 6.2 8 half-maximum activity at pH 6.2 and 8 Salix babylonica