Literature summary extracted from

Kellermann, O.; Brevet, A.; Tonetti, H.; Waller, J.P.
Methionyl-tRNA synthetase from sheep liver. Purification of a fully active monomer derived from high-molecular-weight complexes by trypsin treatment (1978), Eur. J. Biochem., 88, 205-210.

Organism

EC Number	Organism	UniProt	Comment	Textmining
6.1.1.10	Ovis aries	-	-	-

Purification (Commentary)

EC Number	Purification (Comment)	Organism
6.1.1.10	fully active monomeric enzyme derived from high-molecular-weight complexes by controlled proteolysis, trypsin-treatment	Ovis aries

Source Tissue

EC Number	Source Tissue	Comment	Organism	Textmining
6.1.1.10	liver	-	Ovis aries	-

Specific Activity [micromol/min/mg]

EC Number	Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
6.1.1.10	0.675	-	-	Ovis aries

Storage Stability

EC Number	Storage Stability	Organism
6.1.1.10	-20°C, potassium phosphate buffer, pH 7.5, 50% glycerol, 1 mM dithioerythritol, stable for at least 6 months	Ovis aries

Substrates and Products (Substrate)

EC Number	Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
6.1.1.10	ATP + L-methionine + tRNAMet	-	Ovis aries	AMP + diphosphate + L-methionyl-tRNAMet	-	?

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Release 2023.1 (January 2023)