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Literature summary extracted from

  • Kutsuki, H.; Shimada, M.; Higuchi, T.
    Distribution and roles of p-hydroxycinnamate:CoA ligase in lignin biosynthesis (1982), Phytochemistry, 21, 267-271.
No PubMed abstract available

Inhibitors

EC Number Inhibitors Comment Organism Structure
6.2.1.12 Zn2+
-
 Erythrina crista-galli

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
6.2.1.12 0.001
-
 sinapoate
-
 Erythrina crista-galli
6.2.1.12 0.0021
-
 ferulate
-
 Erythrina crista-galli

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
6.2.1.12 Co2+ 97% of the activation relative to Mg2+ Erythrina crista-galli
6.2.1.12 Mg2+ required Erythrina crista-galli
6.2.1.12 Mn2+ 85% of the activation relative to Mg2+ Erythrina crista-galli
6.2.1.12 Ni2+ 81% of the activation relative to Mg2+ Erythrina crista-galli

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
6.2.1.12 40000
-
 gel filtration Erythrina crista-galli

Organism

EC Number Organism UniProt Comment Textmining
6.2.1.12 Erythrina crista-galli
-
-
-
6.2.1.12 Metasequoia glyptostroboides
-
-
-
6.2.1.12 Paulownia tomentosa
-
-
-
6.2.1.12 Phyllostachys bambusoides
-
-
-
6.2.1.12 Platycladus orientalis
-
-
-
6.2.1.12 Populus x canadensis
-
-
-
6.2.1.12 Robinia pseudoacacia
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
6.2.1.12
-
 Erythrina crista-galli

Source Tissue

EC Number Source Tissue Comment Organism Textmining
6.2.1.12 phloem
-
 Erythrina crista-galli
-
6.2.1.12 xylem
-
 Populus x canadensis
-
6.2.1.12 xylem
-
 Erythrina crista-galli
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
6.2.1.12 additional information
-
-
 Erythrina crista-galli

Storage Stability

EC Number Storage Stability Organism
6.2.1.12 -20°C, 10% or 30% glycerol, or 10% ethylene glycol, stable for 10 days Erythrina crista-galli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.2.1.12 ATP + 3,4-dimethoxycinnamate + CoA 84% of the activity relative to ferulate Erythrina crista-galli AMP + diphosphate + 3,4-dimethoxycinnamoyl-CoA
-
 ?
6.2.1.12 ATP + 4-coumarate + CoA
-
 Populus x canadensis AMP + diphosphate + 4-coumaroyl-CoA
-
 ?
6.2.1.12 ATP + 4-coumarate + CoA
-
 Platycladus orientalis AMP + diphosphate + 4-coumaroyl-CoA
-
 ?
6.2.1.12 ATP + 4-coumarate + CoA
-
 Metasequoia glyptostroboides AMP + diphosphate + 4-coumaroyl-CoA
-
 ?
6.2.1.12 ATP + 4-coumarate + CoA
-
 Robinia pseudoacacia AMP + diphosphate + 4-coumaroyl-CoA
-
 ?
6.2.1.12 ATP + 4-coumarate + CoA
-
 Paulownia tomentosa AMP + diphosphate + 4-coumaroyl-CoA
-
 ?
6.2.1.12 ATP + 4-coumarate + CoA
-
 Phyllostachys bambusoides AMP + diphosphate + 4-coumaroyl-CoA
-
 ?
6.2.1.12 ATP + 4-coumarate + CoA 140% of the activity relative to ferulate Erythrina crista-galli AMP + diphosphate + 4-coumaroyl-CoA
-
 ?
6.2.1.12 ATP + 4-methoxycinnamate + CoA 68% of the activity relative to ferulate Erythrina crista-galli AMP + diphosphate + 4-methoxycinnamoyl-CoA
-
 ?
6.2.1.12 ATP + 5-hydroxyferulate + CoA
-
 Populus x canadensis AMP + diphosphate + 5-hydroxyferuloyl-CoA
-
 ?
6.2.1.12 ATP + 5-hydroxyferulate + CoA 17% of the activity relative to ferulate Erythrina crista-galli AMP + diphosphate + 5-hydroxyferuloyl-CoA
-
 ?
6.2.1.12 ATP + caffeate + CoA
-
 Populus x canadensis AMP + diphosphate + caffeoyl-CoA
-
 ?
6.2.1.12 ATP + caffeate + CoA 48% of the activity relative to ferulate Erythrina crista-galli AMP + diphosphate + caffeoyl-CoA
-
 ?
6.2.1.12 ATP + ferulate + CoA
-
 Populus x canadensis AMP + diphosphate + feruloyl-CoA
-
 ?
6.2.1.12 ATP + ferulate + CoA
-
 Erythrina crista-galli AMP + diphosphate + feruloyl-CoA
-
 ?
6.2.1.12 ATP + isoferulate + CoA as effective as ferulate Erythrina crista-galli AMP + diphosphate + isoferuloyl-CoA
-
 ?
6.2.1.12 ATP + sinapoate + CoA
-
 Populus x canadensis AMP + diphosphate + sinapoyl-CoA
-
 ?
6.2.1.12 ATP + sinapoate + CoA
-
 Robinia pseudoacacia AMP + diphosphate + sinapoyl-CoA
-
 ?
6.2.1.12 ATP + sinapoate + CoA
-
 Phyllostachys bambusoides AMP + diphosphate + sinapoyl-CoA
-
 ?
6.2.1.12 ATP + sinapoate + CoA 48% of the activity relative to ferulate Erythrina crista-galli AMP + diphosphate + sinapoyl-CoA
-
 ?
6.2.1.12 additional information most of angiosperm and gymnosperm enzymes are active with ferulate but not with sinapoate (?). The enzymes of Erythrina crista-galli, Robinia pseudoacacia and bamboo show considerable activity with sinapoate Populus x canadensis ?
-
 ?
6.2.1.12 additional information most of angiosperm and gymnosperm enzymes are active with ferulate but not with sinapoate (?). The enzymes of Erythrina crista-galli, Robinia pseudoacacia and bamboo show considerable activity with sinapoate Platycladus orientalis ?
-
 ?
6.2.1.12 additional information most of angiosperm and gymnosperm enzymes are active with ferulate but not with sinapoate (?). The enzymes of Erythrina crista-galli, Robinia pseudoacacia and bamboo show considerable activity with sinapoate Metasequoia glyptostroboides ?
-
 ?
6.2.1.12 additional information most of angiosperm and gymnosperm enzymes are active with ferulate but not with sinapoate (?). The enzymes of Erythrina crista-galli, Robinia pseudoacacia and bamboo show considerable activity with sinapoate Erythrina crista-galli ?
-
 ?
6.2.1.12 additional information most of angiosperm and gymnosperm enzymes are active with ferulate but not with sinapoate (?). The enzymes of Erythrina crista-galli, Robinia pseudoacacia and bamboo show considerable activity with sinapoate Robinia pseudoacacia ?
-
 ?
6.2.1.12 additional information most of angiosperm and gymnosperm enzymes are active with ferulate but not with sinapoate (?). The enzymes of Erythrina crista-galli, Robinia pseudoacacia and bamboo show considerable activity with sinapoate Paulownia tomentosa ?
-
 ?
6.2.1.12 additional information most of angiosperm and gymnosperm enzymes are active with ferulate but not with sinapoate (?). The enzymes of Erythrina crista-galli, Robinia pseudoacacia and bamboo show considerable activity with sinapoate Phyllostachys bambusoides ?
-
 ?

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
6.2.1.12 7.2 7.6
-
 Erythrina crista-galli