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Literature summary extracted from

  • Lhderitz, T.; Schatz, G.; Grisebach, H.
    Enzymic synthesis of lignin precursors. Purification and properties of 4-coumarate:CoA ligase from cambial sap of spruce (Picia abies L.) (1982), Eur. J. Biochem., 123, 583-586.
    View publication on PubMed

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
6.2.1.12 57000
-
 gel filtration Picea abies
6.2.1.12 63000
-
 1 * 63000, SDS-PAGE Picea abies

Organism

EC Number Organism UniProt Comment Textmining
6.2.1.12 Picea abies
-
 isoenzymes not detected
-

Purification (Commentary)

EC Number Purification (Comment) Organism
6.2.1.12
-
 Picea abies

Source Tissue

EC Number Source Tissue Comment Organism Textmining
6.2.1.12 cambium sap Picea abies
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
6.2.1.12 additional information
-
-
 Picea abies

Storage Stability

EC Number Storage Stability Organism
6.2.1.12 -20°C, 10 mM sodium/potassium phosphate, pH 7.0, 42 mM mercaptoethanol, 20% ethyleneglycol, stable for several months Picea abies

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.2.1.12 ATP + 4-coumarate + CoA 94% of the activity relative to ferulate Picea abies AMP + diphosphate + 4-coumaroyl-CoA
-
 ?
6.2.1.12 ATP + 5-hydroxyferulate + CoA 18% of the activity relative to ferulate Picea abies AMP + diphosphate + 5-hydroxyferuloyl-CoA
-
 ?
6.2.1.12 ATP + caffeate + CoA 86% of the activity relative to ferulate Picea abies AMP + diphosphate + caffeoyl-CoA
-
 ?
6.2.1.12 ATP + cinnamate + CoA 14% of the activity relative to ferulate Picea abies AMP + diphosphate + cinnamoyl-CoA
-
 ?
6.2.1.12 ATP + ferulate + CoA
-
 Picea abies AMP + diphosphate + feruloyl-CoA
-
 ?
6.2.1.12 ATP + sinapoate + CoA no activity Picea abies AMP + diphosphate + sinapoyl-CoA
-
 ?

Subunits

EC Number Subunits Comment Organism
6.2.1.12 monomer 1 * 63000, SDS-PAGE Picea abies

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
6.2.1.12 7.5
-
-
 Picea abies

pH Range

EC Number pH Minimum pH Maximum Comment Organism
6.2.1.12 6.8 7.5 6.8: about 80% of maximal activity, 7.5: maximal activity Picea abies