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Literature summary extracted from

  • Knobloch, K.H.; Hahlbrock, K.
    4-Coumarate:CoA ligase from cell suspension cultures of Petroselinum hortense Hoffm. (1977), Arch. Biochem. Biophys., 184, 237-248.
    View publication on PubMed

General Stability

EC Number General Stability Organism
6.2.1.12 complete stabilization in presence of 30% glycerol or 30% ethylene glycol Petroselinum crispum
6.2.1.12 sucrose stabilizes Petroselinum crispum

Inhibitors

EC Number Inhibitors Comment Organism Structure
6.2.1.12 4-chloromercuribenzoate
-
 Petroselinum crispum
6.2.1.12 4-coumarate
-
 Petroselinum crispum
6.2.1.12 CoA
-
 Petroselinum crispum
6.2.1.12 iodoacetamide
-
 Petroselinum crispum
6.2.1.12 NEM
-
 Petroselinum crispum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
6.2.1.12 0.014
-
 caffeate 2-coumarate Petroselinum crispum
6.2.1.12 0.02
-
 ferulate caffeate Petroselinum crispum
6.2.1.12 0.026
-
 isoferulate
-
 Petroselinum crispum
6.2.1.12 0.028
-
 3-coumarate
-
 Petroselinum crispum
6.2.1.12 0.033
-
 ferulate
-
 Petroselinum crispum
6.2.1.12 0.28
-
 cinnamate
-
 Petroselinum crispum
6.2.1.12 0.34
-
 5-hydroxyferulate
-
 Petroselinum crispum
6.2.1.12 0.5
-
 4-methoxyferulate
-
 Petroselinum crispum
6.2.1.12 0.55
-
 3,4-dimethoxycinnamate
-
 Petroselinum crispum

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
6.2.1.12 Ca2+ 33% of the activation compared to Mg2+ Petroselinum crispum
6.2.1.12 Mg2+ required in a molar ratio of 1:1 with ATP Petroselinum crispum
6.2.1.12 Mn2+ 97% of the activation compared to Mg2+ Petroselinum crispum

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
6.2.1.12 67000
-
 gel filtration Petroselinum crispum

Organism

EC Number Organism UniProt Comment Textmining
6.2.1.12 Petroselinum crispum
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
6.2.1.12 partial Petroselinum crispum

Source Tissue

EC Number Source Tissue Comment Organism Textmining
6.2.1.12 cell culture
-
 Petroselinum crispum
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
6.2.1.12 additional information
-
-
 Petroselinum crispum

Storage Stability

EC Number Storage Stability Organism
6.2.1.12 complete loss of activity in crude enzyme extract after 4 days at 4-6°C. 0.92 M sucrose can reduce the loss of activity to 20%. Complete stabilization in presence of 30% glycerol or 30% ethylene glycol Petroselinum crispum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.2.1.12 ATP + 2-coumarate + CoA
-
 Petroselinum crispum AMP + diphosphate + 2-coumaroyl-CoA
-
 ?
6.2.1.12 ATP + 3,4,5-trimethoxycinnamate + CoA no activity Petroselinum crispum AMP + diphosphate + 3,4,5-trimethoxycinnamoyl-CoA
-
 ?
6.2.1.12 ATP + 3,4-dimethoxycinnamate + CoA
-
 Petroselinum crispum AMP + diphosphate + 3,4-dimethoxycinnamoyl-CoA
-
 ?
6.2.1.12 ATP + 3-coumarate + CoA
-
 Petroselinum crispum AMP + diphosphate + 3-coumaroyl-CoA
-
 ?
6.2.1.12 ATP + 4-coumarate + CoA
-
 Petroselinum crispum AMP + diphosphate + 4-coumaroyl-CoA
-
 ?
6.2.1.12 ATP + 4-methoxycinnamate + CoA
-
 Petroselinum crispum AMP + diphosphate + 4-methoxycinnamoyl-CoA
-
 ?
6.2.1.12 ATP + 4-methoxyferulate + CoA
-
 Petroselinum crispum AMP + diphosphate + 4-methoxyferuloyl-CoA
-
 ?
6.2.1.12 ATP + 5-hydroxyferulate + CoA
-
 Petroselinum crispum AMP + diphosphate + 5-hydroxyferuloyl-CoA
-
 ?
6.2.1.12 ATP + caffeate + CoA
-
 Petroselinum crispum AMP + diphosphate + caffeoyl-CoA
-
 ?
6.2.1.12 ATP + cinnamate + CoA
-
 Petroselinum crispum AMP + diphosphate + cinnamoyl-CoA
-
 ?
6.2.1.12 ATP + ferulate + CoA
-
 Petroselinum crispum AMP + diphosphate + feruloyl-CoA
-
 ?
6.2.1.12 ATP + isoferulate + CoA
-
 Petroselinum crispum AMP + diphosphate + isoferuloyl-CoA
-
 ?
6.2.1.12 ATP + sinapoate + CoA no activity Petroselinum crispum AMP + diphosphate + sinapoyl-CoA
-
 ?
6.2.1.12 additional information absolute requirement for ATP Petroselinum crispum ?
-
 ?
6.2.1.12 additional information ATP-diphosphate exchange reaction is activated by cinnamate, dihydro-4-coumarate, caprylate and malonate Petroselinum crispum ?
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
6.2.1.12 35
-
-
 Petroselinum crispum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
6.2.1.12 7 8 ATP-diphosphate exchange Petroselinum crispum
6.2.1.12 7.8 8 formation of 4-coumaroyl-CoA Petroselinum crispum