EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
6.2.1.12 | 4-coumarate | above 0.1 mM | Glycine max | |
6.2.1.12 | CoASH | above 0.1 mM | Glycine max | |
6.2.1.12 | Ionic strength | activity of isoenzyme 1 decreases steadily with increasing ionic strength at least in the range from 0.1 to 0.8 M Tris-HCl, highest rate of isoenzyme 2 activity is obtained at concentrations of about 0.3 M Tris-HCl | Glycine max |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
6.2.1.12 | 0.004 | - |
CoASH | isoenzyme 2 | Glycine max | |
6.2.1.12 | 0.007 | - |
CoASH | isoenzyme 1 | Glycine max | |
6.2.1.12 | 0.009 | - |
ferulate | isoenzyme 1 | Glycine max | |
6.2.1.12 | 0.011 | - |
sinapoate | isoenzyme 1 | Glycine max | |
6.2.1.12 | 0.012 | - |
5-hydroxyferulate | isoenzyme 1 | Glycine max | |
6.2.1.12 | 0.014 | - |
caffeate | isoenzyme 2 | Glycine max | |
6.2.1.12 | 0.017 | - |
4-coumarate | isoenzyme 2 | Glycine max | |
6.2.1.12 | 0.03 | - |
3-coumarate | isoenzyme 2 | Glycine max | |
6.2.1.12 | 0.032 | - |
4-coumarate | isoenzyme 1 | Glycine max | |
6.2.1.12 | 0.04 | - |
caffeate | isoenzyme 1 | Glycine max | |
6.2.1.12 | 0.065 | - |
ATP | isoenzyme 2 | Glycine max | |
6.2.1.12 | 0.09 | - |
2-coumarate | isoenzyme 2 | Glycine max | |
6.2.1.12 | 0.1 | - |
isoferulate | 3,4-dimethoxycinnamate, , isoenzyme 1 | Glycine max | |
6.2.1.12 | 0.12 | - |
5-hydroxyferulate | isoenzyme 2 | Glycine max | |
6.2.1.12 | 0.13 | - |
ferulate | isoenzyme 2 | Glycine max | |
6.2.1.12 | 0.15 | - |
isoferulate | isoenzyme 2 | Glycine max | |
6.2.1.12 | 0.17 | - |
ATP | 3-coumarate, , isoenzyme 1 | Glycine max | |
6.2.1.12 | 0.18 | - |
2-coumarate | isoenzyme 1 | Glycine max | |
6.2.1.12 | 0.23 | - |
3,4,5-trimethoxycinnamate | isoenzyme 1 | Glycine max | |
6.2.1.12 | 0.38 | - |
4-methoxycinnamate | isoenzyme 1 | Glycine max | |
6.2.1.12 | 1.3 | - |
cinnamate | isoenzyme 1 | Glycine max | |
6.2.1.12 | 4.5 | - |
cinnamate | isoenzyme 2 | Glycine max |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
6.2.1.12 | Co2+ | divalent cation required, Mg2+, Mn2+ and Co2+ are equally effective | Glycine max | |
6.2.1.12 | Mg2+ | divalent cation required, Mg2+, Mn2+ and Co2+ are equally effective | Glycine max | |
6.2.1.12 | Mn2+ | divalent cation required, Mg2+, Mn2+ and Co2+ are equally effective | Glycine max |
EC Number | Organic Solvent | Comment | Organism |
---|---|---|---|
6.2.1.12 | Ethylene glycol | 30% ethylene glycol stabilizes. Loss of activity after 7 days is reduced from 80% to about 25% | Glycine max |
6.2.1.12 | Glycerol | 30% glycerol stabilizes. Loss of activity after 7 days is reduced from 80% to about 25% | Glycine max |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
6.2.1.12 | Glycine max | - |
var. Mandarin | - |
6.2.1.12 | Glycine max | - |
2 isoenzymes | - |
EC Number | Purification (Comment) | Organism |
---|---|---|
6.2.1.12 | partial | Glycine max |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
6.2.1.12 | cell culture | - |
Glycine max | - |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
6.2.1.12 | additional information | - |
- |
Glycine max |
EC Number | Storage Stability | Organism |
---|---|---|
6.2.1.12 | crude enzyme preparation is unstable in buffered aqueous solution at temperatures at or above 0°C | Glycine max |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
6.2.1.12 | ATP + 2-coumarate + CoA | - |
Glycine max | AMP + diphosphate + 2-coumaroyl-CoA | - |
? | |
6.2.1.12 | ATP + 3,4,5-trimethoxycinnamate + CoA | isoenzyme 1 is active, isoenzyme 2 not | Glycine max | AMP + diphosphate + 3,4,5-trimethoxycinnamoyl-CoA | - |
? | |
6.2.1.12 | ATP + 3,4-dimethoxycinnamate + CoA | isoenzyme 1 is active, isoenzyme 2 not | Glycine max | AMP + diphosphate + 3,4-dimethoxycinnamoyl-CoA | - |
? | |
6.2.1.12 | ATP + 3-coumarate + CoA | - |
Glycine max | AMP + diphosphate + 3-coumaroyl-CoA | - |
? | |
6.2.1.12 | ATP + 4-coumarate + CoA | - |
Glycine max | AMP + diphosphate + 4-coumaroyl-CoA | - |
? | |
6.2.1.12 | ATP + 4-methoxycinnamate + CoA | isoenzyme 1 is active, isoenzyme 2 not | Glycine max | AMP + diphosphate + 4-methoxycinnamoyl-CoA | - |
? | |
6.2.1.12 | ATP + 5-hydroxyferulate + CoA | - |
Glycine max | AMP + diphosphate + 5-hydroxyferuloyl-CoA | - |
? | |
6.2.1.12 | ATP + caffeate + CoA | - |
Glycine max | AMP + diphosphate + caffeoyl-CoA | - |
? | |
6.2.1.12 | ATP + cinnamate + CoA | - |
Glycine max | AMP + diphosphate + cinnamoyl-CoA | - |
? | |
6.2.1.12 | ATP + ferulate + CoA | - |
Glycine max | AMP + diphosphate + feruloyl-CoA | - |
? | |
6.2.1.12 | ATP + isoferulate + CoA | - |
Glycine max | AMP + diphosphate + isoferuloyl-CoA | - |
? | |
6.2.1.12 | ATP + sinapoate + CoA | isoenzyme 1 is active with sinapoate, isoenzyme 2 not | Glycine max | AMP + diphosphate + sinapoyl-CoA | - |
? |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
6.2.1.12 | 7.7 | - |
formation of 4-coumaroyl-CoA, isoenzyme 2 | Glycine max |
6.2.1.12 | 8.3 | - |
formation of 4-coumaroyl-CoA, isoenzyme 1 | Glycine max |