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Literature summary extracted from

  • Gross, G.G.; Zenk, M.H.
    Isolation and properties of hydroxycinnamate:CoA ligase from lignifying tissues of Forsythia (1974), Eur. J. Biochem., 42, 453-459.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
6.2.1.12 CoA substrate inhibition Forsythia suspensa
6.2.1.12 ferulate strong substrate inhibition above 0.2 mM Forsythia suspensa

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
6.2.1.12 0.0032
-
 CoA
-
 Forsythia suspensa
6.2.1.12 0.02
-
 ferulate
-
 Forsythia suspensa
6.2.1.12 0.036
-
 ATP
-
 Forsythia suspensa

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
6.2.1.12 Mg2+
-
 Forsythia suspensa

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
6.2.1.12 55000
-
 gel filtration Forsythia suspensa

Organism

EC Number Organism UniProt Comment Textmining
6.2.1.12 Forsythia suspensa
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
6.2.1.12 partial Forsythia suspensa

Source Tissue

EC Number Source Tissue Comment Organism Textmining
6.2.1.12 stem young, lignifying Forsythia suspensa
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
6.2.1.12 additional information
-
-
 Forsythia suspensa

Storage Stability

EC Number Storage Stability Organism
6.2.1.12 -20°C, 50% glycerol Forsythia suspensa

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.2.1.12 ATP + 4-coumarate + CoA
-
 Forsythia suspensa AMP + diphosphate + 4-coumaroyl-CoA
-
 ?
6.2.1.12 ATP + ferulate + CoA
-
 Forsythia suspensa AMP + diphosphate + feruloyl-CoA
-
 ?
6.2.1.12 CTP + ferulate + CoA 7.7% of the activity relative to ATP Forsythia suspensa CMP + diphosphate + feruloyl-CoA
-
 ?
6.2.1.12 GTP + ferulate + CoA 5.7% of the activity relative to ATP Forsythia suspensa GMP + diphosphate + feruloyl-CoA
-
 ?
6.2.1.12 ITP + ferulate + CoA 20.2% of the activity relative to ATP Forsythia suspensa IMP + diphosphate + feruloyl-CoA
-
 ?
6.2.1.12 additional information in ATP-diphosphate exchange reaction absolute specificity for cinnamate Forsythia suspensa ?
-
 ?
6.2.1.12 UTP + ferulate + CoA 12.5% of the activity relative to GTP Forsythia suspensa UMP + diphosphate + feruloyl-CoA
-
 ?

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
6.2.1.12 47
-
 rapid inactivation Forsythia suspensa

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
6.2.1.12 7
-
 ATP-diphosphate exchange reaction Forsythia suspensa
6.2.1.12 7.3
-
 formation of feruloyl-CoA Forsythia suspensa

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
6.2.1.12 6 8.5 stable Forsythia suspensa