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Literature summary extracted from

  • Goda, S.K.; Minton, N.P.; Botting, N.P.; Gani, D.
    Cloning, sequencing, and expression in Escherichia coli of the Clostridium tetanomorphum gene encoding beta-methylaspartase and characterization of the recombinant protein (1992), Biochemistry, 31, 10747-10756.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.3.1.2 expression in Escherichia coli Clostridium tetanomorphum

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
4.3.1.2 K+
-
Clostridium tetanomorphum

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.3.1.2 45539
-
2 * 45539, calculation from sequence of amino acid Clostridium tetanomorphum
4.3.1.2 49000
-
2 * 49000, SDS-PAGE Clostridium tetanomorphum

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.3.1.2 L-threo-3-methylaspartate Clostridium tetanomorphum
-
?
-
?
4.3.1.2 L-threo-3-methylaspartate Clostridium tetanomorphum H1 / ATCC 15920
-
?
-
?
4.3.1.2 additional information Clostridium tetanomorphum enzyme is involved in the catabolic pathway for glutamate ?
-
?
4.3.1.2 additional information Clostridium tetanomorphum H1 / ATCC 15920 enzyme is involved in the catabolic pathway for glutamate ?
-
?

Organism

EC Number Organism UniProt Comment Textmining
4.3.1.2 Clostridium tetanomorphum
-
-
-
4.3.1.2 Clostridium tetanomorphum
-
ATCC 15920
-
4.3.1.2 Clostridium tetanomorphum H1 / ATCC 15920
-
-
-

Reaction

EC Number Reaction Comment Organism Reaction ID
4.3.1.2 L-threo-3-methylaspartate = mesaconate + NH3 mechanism Clostridium tetanomorphum

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.3.1.2 141
-
-
Clostridium tetanomorphum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.3.1.2 erythro-3-methylaspartate
-
Clostridium tetanomorphum ?
-
?
4.3.1.2 erythro-3-methylaspartate
-
Clostridium tetanomorphum H1 / ATCC 15920 ?
-
?
4.3.1.2 L-aspartate (2S)-aspartate Clostridium tetanomorphum fumarate + NH3
-
?
4.3.1.2 L-aspartate (2S)-aspartate Clostridium tetanomorphum H1 / ATCC 15920 fumarate + NH3
-
?
4.3.1.2 L-threo-3-methylaspartate r Clostridium tetanomorphum mesaconate + NH3
-
?
4.3.1.2 L-threo-3-methylaspartate r Clostridium tetanomorphum H1 / ATCC 15920 mesaconate + NH3
-
?
4.3.1.2 L-threo-3-methylaspartate
-
Clostridium tetanomorphum ?
-
?
4.3.1.2 L-threo-3-methylaspartate
-
Clostridium tetanomorphum H1 / ATCC 15920 ?
-
?
4.3.1.2 additional information enzyme is involved in the catabolic pathway for glutamate Clostridium tetanomorphum ?
-
?
4.3.1.2 additional information enzyme is involved in the catabolic pathway for glutamate Clostridium tetanomorphum H1 / ATCC 15920 ?
-
?

Subunits

EC Number Subunits Comment Organism
4.3.1.2 dimer 2 * 49000, SDS-PAGE Clostridium tetanomorphum
4.3.1.2 dimer 2 * 45539, calculation from sequence of amino acid Clostridium tetanomorphum