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Literature summary extracted from

  • Kawata, Y.; Tamura, K.; Yano, S.; Mizobata, T.; Nagai, J.; Esaki, N.; Soda, K.; Tokushige, M.; Yumoto, N.
    Purification and characterization of thermostable aspartase from Bacillus sp. YM55-1 (1999), Arch. Biochem. Biophys., 366, 40-46.
    View publication on PubMed

General Stability

EC Number General Stability Organism
4.3.1.1 1 M guanidine hydrochloride, 80% residual activity Bacillus sp. (in: Bacteria)
4.3.1.1 after incubation in the presence of guanidine hydrochloride at a concentration of 1.1 M 50% loss of activity after 60 min Bacillus sp. (in: Bacteria)
4.3.1.1 after incubation in the presence of guanidine hydrochloride at a concentration of 1.1 M complete inactivation Escherichia coli

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.3.1.1 2.3
-
 L-aspartate 30°C Pseudomonas fluorescens
4.3.1.1 2.8
-
 L-aspartate 30°C Escherichia coli
4.3.1.1 28.5
-
 L-aspartate 30°C Bacillus sp. (in: Bacteria)
4.3.1.1 28.5
-
 L-aspartate 30°C, pH 8.5 Bacillus sp. (in: Bacteria)
4.3.1.1 32
-
 L-aspartate 55°C, pH 7.8 Bacillus sp. (in: Bacteria)
4.3.1.1 32
-
 L-aspartate 55°C Bacillus sp. (in: Bacteria)

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
4.3.1.1 additional information no activation by Mg2+ at alkaline pH Bacillus sp. (in: Bacteria)

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.3.1.1 51000
-
 4 * 51000, SDS-PAGE Bacillus sp. (in: Bacteria)
4.3.1.1 200000
-
 gel filtration Bacillus sp. (in: Bacteria)

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.3.1.1 additional information Escherichia coli
-
 ?
-
 ?
4.3.1.1 additional information Pseudomonas fluorescens
-
 ?
-
 ?
4.3.1.1 additional information Bacillus sp. (in: Bacteria) important role in the bacterial nitrogen metabolism ?
-
 ?
4.3.1.1 additional information Bacillus sp. (in: Bacteria) YM55-1 important role in the bacterial nitrogen metabolism ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
4.3.1.1 Bacillus sp. (in: Bacteria)
-
-
-
4.3.1.1 Bacillus sp. (in: Bacteria)
-
 YM55-1
-
4.3.1.1 Bacillus sp. (in: Bacteria) YM55-1
-
-
-
4.3.1.1 Bacillus sp. (in: Bacteria) YM55-1
-
 YM55-1
-
4.3.1.1 Escherichia coli
-
-
-
4.3.1.1 Pseudomonas fluorescens
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.3.1.1
-
 Bacillus sp. (in: Bacteria)

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.3.1.1 additional information
-
-
 Escherichia coli
4.3.1.1 additional information
-
-
 Bacillus sp. (in: Bacteria)
4.3.1.1 167
-
 30°C Escherichia coli
4.3.1.1 214
-
 30°C Pseudomonas fluorescens
4.3.1.1 700
-
 30°C Bacillus sp. (in: Bacteria)
4.3.1.1 800
-
 55C, pH 8.0 Escherichia coli
4.3.1.1 2200
-
 55C Bacillus sp. (in: Bacteria)
4.3.1.1 2500
-
 65C, pH 7.5 Bacillus sp. (in: Bacteria)

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.3.1.1 L-aspartate
-
 Bacillus sp. (in: Bacteria) fumarate + NH3
-
 ?
4.3.1.1 L-aspartate r Escherichia coli fumarate + NH3
-
 ?
4.3.1.1 L-aspartate r Pseudomonas fluorescens fumarate + NH3
-
 ?
4.3.1.1 L-aspartate r Bacillus sp. (in: Bacteria) fumarate + NH3
-
 ?
4.3.1.1 L-aspartate natural substrate Escherichia coli fumarate + NH3
-
 ?
4.3.1.1 L-aspartate natural substrate Pseudomonas fluorescens fumarate + NH3
-
 ?
4.3.1.1 L-aspartate natural substrate Bacillus sp. (in: Bacteria) fumarate + NH3
-
 ?
4.3.1.1 L-aspartate
-
 Bacillus sp. (in: Bacteria) YM55-1 fumarate + NH3
-
 ?
4.3.1.1 L-aspartate r Bacillus sp. (in: Bacteria) YM55-1 fumarate + NH3
-
 ?
4.3.1.1 L-aspartate natural substrate Bacillus sp. (in: Bacteria) YM55-1 fumarate + NH3
-
 ?
4.3.1.1 additional information
-
 Escherichia coli ?
-
 ?
4.3.1.1 additional information
-
 Pseudomonas fluorescens ?
-
 ?
4.3.1.1 additional information
-
 Bacillus sp. (in: Bacteria) ?
-
 ?
4.3.1.1 additional information important role in the bacterial nitrogen metabolism Bacillus sp. (in: Bacteria) ?
-
 ?
4.3.1.1 additional information
-
 Bacillus sp. (in: Bacteria) YM55-1 ?
-
 ?
4.3.1.1 additional information important role in the bacterial nitrogen metabolism Bacillus sp. (in: Bacteria) YM55-1 ?
-
 ?

Subunits

EC Number Subunits Comment Organism
4.3.1.1 tetramer
-
 Escherichia coli
4.3.1.1 tetramer
-
 Pseudomonas fluorescens
4.3.1.1 tetramer 4 * 51000, SDS-PAGE Bacillus sp. (in: Bacteria)

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
4.3.1.1 30
-
 assay at Escherichia coli
4.3.1.1 30
-
 assay at Bacillus sp. (in: Bacteria)
4.3.1.1 55
-
 assay at Bacillus sp. (in: Bacteria)
4.3.1.1 55
-
 at pH 8.0 Escherichia coli
4.3.1.1 65
-
 at pH 7.5 Bacillus sp. (in: Bacteria)

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
4.3.1.1 additional information
-
-
 Escherichia coli
4.3.1.1 additional information
-
-
 Bacillus sp. (in: Bacteria)

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
4.3.1.1 additional information
-
-
 Escherichia coli
4.3.1.1 additional information
-
 moderate thermophile Bacillus sp. (in: Bacteria)
4.3.1.1 55
-
 20% loss of activity after 60 min Bacillus sp. (in: Bacteria)
4.3.1.1 55
-
 complete loss of activity after 60 min Escherichia coli
4.3.1.1 55
-
 60 min, 80% residual activity Bacillus sp. (in: Bacteria)
4.3.1.1 75
-
 5 min, stable Bacillus sp. (in: Bacteria)

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.3.1.1 additional information
-
-
 Escherichia coli
4.3.1.1 additional information
-
-
 Bacillus sp. (in: Bacteria)
4.3.1.1 7.5
-
-
 Escherichia coli
4.3.1.1 7.5
-
 assay at 60C Bacillus sp. (in: Bacteria)
4.3.1.1 8.5
-
-
 Escherichia coli
4.3.1.1 8.5
-
 assay at 30C Bacillus sp. (in: Bacteria)

pH Range

EC Number pH Minimum pH Maximum Comment Organism
4.3.1.1 additional information
-
-
 Escherichia coli
4.3.1.1 additional information
-
-
 Bacillus sp. (in: Bacteria)