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Literature summary extracted from

  • Rosa, M.D.; Sigler, P.B.
    Isolation and characterization of two methionine:tRNA ligases from wheat germ (1977), Eur. J. Biochem., 78, 141-151.
    View publication on PubMed

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
6.1.1.10 0.018
-
 Met ligase A and B Triticum aestivum
6.1.1.10 0.3
-
 ATP ligase B Triticum aestivum
6.1.1.10 0.6
-
 ATP ligase A Triticum aestivum

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
6.1.1.10 Mg2+
-
 Triticum aestivum

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
6.1.1.10 63000
-
 ligase B, sucrose density gradient centrifugation Triticum aestivum
6.1.1.10 70000
-
 1 * 70000, ligase B, SDS-PAGE Triticum aestivum
6.1.1.10 90000
-
 enzyme form A, sucrose density gradient centrifugation Triticum aestivum
6.1.1.10 105000
-
 1 * 105000, ligase A, SDS-PAGE Triticum aestivum

Organism

EC Number Organism UniProt Comment Textmining
6.1.1.10 Triticum aestivum
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
6.1.1.10 two forms: ligase A and B Triticum aestivum

Source Tissue

EC Number Source Tissue Comment Organism Textmining
6.1.1.10 germ
-
 Triticum aestivum
-

Storage Stability

EC Number Storage Stability Organism
6.1.1.10 -20°C, 50% v/v glycerol, stable for several months Triticum aestivum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.1.1.10 ATP + L-methionine + tRNAMet
-
 Triticum aestivum AMP + diphosphate + L-methionyl-tRNAMet
-
 ?

Subunits

EC Number Subunits Comment Organism
6.1.1.10 monomer 1 * 70000, ligase B, SDS-PAGE Triticum aestivum
6.1.1.10 monomer 1 * 105000, ligase A, SDS-PAGE Triticum aestivum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
6.1.1.10 8 8.5
-
 Triticum aestivum