EC Number | Cloned (Comment) | Organism |
---|---|---|
4.2.1.75 | expression in Escherichia coli | Bacillus subtilis |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
4.2.1.75 | Butane-2,3-dione | - |
Bacillus subtilis | |
4.2.1.75 | diethyl dicarbonate | - |
Bacillus subtilis | |
4.2.1.75 | NaN3 | 60% residual activity with 5.0 mM | Bacillus subtilis | |
4.2.1.75 | Phenylglyoxal | - |
Bacillus subtilis | |
4.2.1.75 | pyridoxal 5'-phosphate | Pyridoxal 5'-phosphate and subsequent treatment with NaBH4 | Bacillus subtilis |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
4.2.1.75 | 0.00033 | - |
Hydroxymethylbilane | - |
Bacillus subtilis |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
4.2.1.75 | additional information | bivalent ions, such as MgCl2 up to 20 mM, fail to stimulate enzyme activity | Bacillus subtilis |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
4.2.1.75 | 29100 | - |
nucleotide sequence | Bacillus subtilis |
4.2.1.75 | 32000 | - |
SDS-PAGE | Bacillus subtilis |
4.2.1.75 | 41500 | - |
gel filtration | Bacillus subtilis |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
4.2.1.75 | Bacillus subtilis | - |
- |
- |
EC Number | Oxidation Stability | Organism |
---|---|---|
4.2.1.75 | photooxidation in presence of Rose Bengal | Bacillus subtilis |
EC Number | Purification (Comment) | Organism |
---|---|---|
4.2.1.75 | expression in Escherichia coli | Bacillus subtilis |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
4.2.1.75 | 9.4 | - |
hydroxymethylbilane | Bacillus subtilis |
EC Number | Storage Stability | Organism |
---|---|---|
4.2.1.75 | -70¦C, at least two years | Bacillus subtilis |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
4.2.1.75 | Hydroxymethylbilane | - |
Bacillus subtilis | Uroporphyrinogen III | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
4.2.1.75 | monomer | SDS-PAGE, gel filtration, nucleotide sequence | Bacillus subtilis |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
4.2.1.75 | 45 | - |
- |
Bacillus subtilis |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
4.2.1.75 | additional information | - |
more thermostable than enzymes from other sources | Bacillus subtilis |
4.2.1.75 | 30 | - |
retention of 100% activity after 24 h | Bacillus subtilis |
4.2.1.75 | 45 | - |
retention of 90% activity after 24 h | Bacillus subtilis |
4.2.1.75 | 60 | - |
completely inactivated in 1 min | Bacillus subtilis |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
4.2.1.75 | 4.6 | - |
Hydroxymethylbilane | - |
Bacillus subtilis |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
4.2.1.75 | 8.3 | - |
- |
Bacillus subtilis |