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Literature summary extracted from

  • Frydman, R.B.; Feinstein, G.
    Studies on porphobilinogen deaminase and uroporphyrinogen III cosynthase from human erythrocytes (1974), Biochim. Biophys. Acta, 350, 358-373.
    View publication on PubMed

General Stability

EC Number General Stability Organism
2.5.1.61 phosphate stabilizes Homo sapiens
4.2.1.75 dithiothreitol stabilizes Homo sapiens
4.2.1.75 human erythrocyte enzyme more stable than wheat germ enzyme Homo sapiens

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.5.1.61 2-methylopsopyrroledicarboxylic acid
-
 Homo sapiens
2.5.1.61 Ca2+ 25 mM CaCl2, 90% inhibition Homo sapiens
2.5.1.61 Cd2+ 12 mM, complete inhibition Homo sapiens
2.5.1.61 glycerol 15% inhibits 40% of enzyme activity Homo sapiens
2.5.1.61 isoporphobilinogen
-
 Homo sapiens
2.5.1.61 Mg2+ 25 mM, 90% inhibition Homo sapiens
2.5.1.61 N-ethylmaleimide
-
 Homo sapiens
2.5.1.61 p-hydroxymercuribenzoate
-
 Homo sapiens
4.2.1.75 hydroxylamine
-
 Homo sapiens
4.2.1.75 N-ethylmaleimide
-
 Homo sapiens
4.2.1.75 NH4+
-
 Homo sapiens
4.2.1.75 p-hydroxymercuribenzoate
-
 Homo sapiens
4.2.1.75 sulphydryl reagents
-
 Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.5.1.61 0.046
-
 porphobilinogen porphobilinogen deaminase/uroporphyrinogen III cosynthase enzyme system Homo sapiens
2.5.1.61 0.051
-
 Hydroxymethylbilane porphobilinogen deaminase/uroporphyrinogen III cosynthase enzyme system Homo sapiens
2.5.1.61 0.077
-
 Hydroxymethylbilane
-
 Homo sapiens
2.5.1.61 0.13
-
 porphobilinogen
-
 Homo sapiens

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.5.1.61 25000
-
 gel filtration Homo sapiens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.5.1.61 porphobilinogen Homo sapiens
-
 ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.5.1.61 Homo sapiens
-
-
-
4.2.1.75 Homo sapiens
-
-
-

Oxidation Stability

EC Number Oxidation Stability Organism
2.5.1.61 photooxidation of the enzyme in the presence of methylene blue, 0.03%, and roes bengal, 0.03%, over a 5 min period, inhibits 90% and 60% of the enzyme activity, respectively Homo sapiens
4.2.1.75 not affected by N-bromosuccinimide oxidation Homo sapiens
4.2.1.75 photooxidation in presence of Rose Bengal Homo sapiens

Purification (Commentary)

EC Number Purification (Comment) Organism
2.5.1.61 partial Homo sapiens
4.2.1.75 partial Homo sapiens

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.5.1.61 erythrocyte
-
 Homo sapiens
-
4.2.1.75 erythrocyte
-
 Homo sapiens
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.5.1.61 additional information
-
-
 Homo sapiens

Storage Stability

EC Number Storage Stability Organism
2.5.1.61 -15°C, purified enzyme rapidely loses its activity Homo sapiens
2.5.1.61 4°C, purified enzyme stable for more than a month Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.5.1.61 4 porphobilinogen in the presence of uroporphyrinogen III cosynthase, EC 4.2.1.75 Homo sapiens uroporphyrinogen III + 4 NH3
-
 ?
2.5.1.61 4 porphobilinogen + H2O PBG Homo sapiens hydroxylmethylbilane + 4 NH3
-
 ?
2.5.1.61 porphobilinogen
-
 Homo sapiens ?
-
 ?
4.2.1.75 Hydroxymethylbilane
-
 Homo sapiens Uroporphyrinogen III
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.5.1.61 porphobilinogen deaminase
-
 Homo sapiens

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.5.1.61 45
-
 activity twice that at 37°C Homo sapiens

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.5.1.61 additional information
-
-
 Homo sapiens
2.5.1.61 65
-
 15 min, no loss of activity Homo sapiens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.5.1.61 7.4
-
 phosphate buffer Homo sapiens
2.5.1.61 8.2
-
 Tris-HCl buffer Homo sapiens

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.5.1.61 5 9 pH 5.0: unstable below, pH 9.0 stable above Homo sapiens