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Literature summary extracted from

  • Hart, G.J.; Battersby, A.R.
    Purification and properties of uroporphyrinogen III synthase (co-synthase) from Euglena gracilis (1985), Biochem. J., 232, 151-160.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
4.2.1.75 additional information no bound cofactor Euglena gracilis

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.2.1.75 Butane-2,3-dione 20% residual activity after 45 min at 40 mM Euglena gracilis
4.2.1.75 diethyl dicarbonate 10% residual activity after 5 min at 0.41 mM Euglena gracilis
4.2.1.75 NaN3 25-30% residual activity with 1.0 mM Euglena gracilis
4.2.1.75 Phenylglyoxal 50% residual activity after 1 min at 0.3 mM Euglena gracilis
4.2.1.75 pyridoxal 5'-phosphate
-
 Euglena gracilis
4.2.1.75 uroporphyrinogen III product inhibition Euglena gracilis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.2.1.75 0.012 0.04 Hydroxymethylbilane
-
 Euglena gracilis

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
4.2.1.75 additional information no metal ions present in enzyme Euglena gracilis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.2.1.75 31000
-
 SDS-PAGE Euglena gracilis
4.2.1.75 38500
-
 SDS-PAGE, gel filtration Euglena gracilis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.2.1.75 Hydroxymethylbilane Euglena gracilis synthesis of uroporphyrinogen III, key intermediate for biosynthesis of tetrapyrrolic compounds like chlorophylls, hemes, cytochromes and vitamin B12 ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
4.2.1.75 Euglena gracilis
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.2.1.75
-
 Euglena gracilis

Source Tissue

EC Number Source Tissue Comment Organism Textmining

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.2.1.75 additional information
-
-
 Euglena gracilis

Storage Stability

EC Number Storage Stability Organism
4.2.1.75 -70¦C, several weeks Euglena gracilis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.2.1.75 Hydroxymethylbilane
-
 Euglena gracilis Uroporphyrinogen III
-
 ?
4.2.1.75 Hydroxymethylbilane synthesis of uroporphyrinogen III, key intermediate for biosynthesis of tetrapyrrolic compounds like chlorophylls, hemes, cytochromes and vitamin B12 Euglena gracilis ?
-
 ?

Subunits

EC Number Subunits Comment Organism
4.2.1.75 monomer SDS-PAGE, gel filtration Euglena gracilis

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
4.2.1.75 25
-
 enzyme assay at Euglena gracilis