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Literature summary extracted from

  • Klein, D.; Pohlentz, G.; Schwarzmann, G.; Sandhoff, K.
    Substrate specificity of GM2 and GD3 synthase of Golgi vesicles derived from rat liver (1987), Eur. J. Biochem., 167, 417-424.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.4.1.92 Detergents requirement, solubilized enzyme, e.g. Triton X-100 Rattus norvegicus
2.4.1.92 phosphatidylglycerol activation, detergent-free assay Rattus norvegicus
2.4.1.92 Triton X-100
-
 Rattus norvegicus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.4.1.92 Golgi apparatus
-
 Rattus norvegicus 5794
-
2.4.3.8 Golgi apparatus
-
 Rattus norvegicus 5794
-
2.4.3.8 membrane
-
 Rattus norvegicus 16020
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.4.3.8 CMP-alpha-N-acetylneuraminate + alpha-N-acetylneuraminyl-2,3-beta-D-galactosyl-1,4-beta-D-glucosylceramide Rattus norvegicus branch-point enzyme in ganglioside biosynthetic sequence CMP + alpha-N-acetylneuraminyl-2,8-alpha-N-acetylneuraminyl-2,3-beta-D-galactosyl-1,4-beta-D-glucosylceramide
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.92 Rattus norvegicus
-
-
-
2.4.3.8 Rattus norvegicus
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.4.1.92 liver
-
 Rattus norvegicus
-
2.4.3.8 liver
-
 Rattus norvegicus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.4.3.8 0.00028 0.00055
-
 Rattus norvegicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.92 additional information acceptor specificity Rattus norvegicus ?
-
 ?
2.4.1.92 UDP-N-acetyl-D-galactosamine + (N-acetylneuraminyl)-D-galactosyl-D-glucosylceramide
-
 Rattus norvegicus UDP + N-acetyl-D-galactosaminyl-(N-acetylneuraminyl)-D-galactosyl-D-glucosylceramide i.e. GM2(N-acetylneuraminic acid)-ganglioside ?
2.4.3.8 CMP-alpha-N-acetylneuraminate + alpha-N-acetylneuraminyl-2,3-beta-D-galactosyl-1,4-beta-D-glucosylceramide branch-point enzyme in ganglioside biosynthetic sequence Rattus norvegicus CMP + alpha-N-acetylneuraminyl-2,8-alpha-N-acetylneuraminyl-2,3-beta-D-galactosyl-1,4-beta-D-glucosylceramide
-
 ?
2.4.3.8 CMP-N-acetylneuraminate + N-alpha-acetylneuraminyl-2,3-beta-D-galactosyl-1,4-beta-D-glucosylceramide i.e. sialosyllactosylceramide or ganglioside GM3, specificity is determined by the substrate's negative charge and the acyl-residue in amide bond to the amino group of neuraminic acid Rattus norvegicus CMP + alpha-N-acetylneuraminyl-2,8-alpha-N-acetylneuraminyl-2,3-beta-D-galactosyl-1,4-beta-D-glucosylceramide i.e. disialosyllactosylceramide or ganglioside GD3 ?
2.4.3.8 CMP-N-acetylneuraminate + N-alpha-acetylneuraminyl-2,3-beta-D-galactosyl-1,4-beta-D-glucosylceramide poor substrates are GM3 methyl ester, GM3 amide or GM3 methyl amide, no substrates are neuraminyllactosylceramide or N-biotinylneuraminyllactosylceramide Rattus norvegicus CMP + alpha-N-acetylneuraminyl-2,8-alpha-N-acetylneuraminyl-2,3-beta-D-galactosyl-1,4-beta-D-glucosylceramide i.e. disialosyllactosylceramide or ganglioside GD3 ?
2.4.3.8 CMP-N-acetylneuraminate + N-butyrylneuraminyl-alpha-2,3-galactosyl-beta-1,4-glucosyl-beta-1,1-ceramide sialylated at about 60% the rate of GM3 Rattus norvegicus CMP + ?
-
 ?
2.4.3.8 CMP-N-acetylneuraminate + N-glycolylneuraminyl-alpha-2,3-galactosyl-beta-1,4-glucosyl-beta-1,1-ceramide
-
 Rattus norvegicus CMP + ?
-
 ?
2.4.3.8 CMP-N-acetylneuraminate + N-lyso-GM3 N-acetyl derivative is a better substrate than GM3, detergent-like effect Rattus norvegicus CMP + N-lyso-GD3
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.4.1.92 37
-
 assay at Rattus norvegicus
2.4.3.8 37
-
 assay at Rattus norvegicus