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Literature summary extracted from
- Purification and characterization of 3alpha-hydroxysteroid dehydrogenase from chicken hepatic cytosol (1990), J. Steroid Biochem. Mol. Biol., 37, 569-574.
General Stability
| EC Number | General Stability | Organism |
|---|---|---|
| 1.1.1.50 | not stable at 4°C | Gallus gallus |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.1.1.50 | 0.0016 | - |
5beta-androstan-3,17-dione | - |
Gallus gallus |  |
Localization
| EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|---|
| 1.1.1.50 | cytosol | - |
Gallus gallus | 5829 | - |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 1.1.1.50 | 66000 | - |
gel filtration | Gallus gallus |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.1.1.50 | Gallus gallus | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 1.1.1.50 | - |
Gallus gallus |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 1.1.1.50 | liver | - |
Gallus gallus | - |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 1.1.1.50 | 0.00024 | - |
purified enzyme, substrate 5beta-androstan-3,17-dione | Gallus gallus |
Storage Stability
| EC Number | Storage Stability | Organism |
|---|---|---|
| 1.1.1.50 | -20°C to -80°C, plus glycerol and 2-mercaptoethanol | Gallus gallus |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.1.1.50 | 5beta-androstan-3,17-dione + NAD(P)H | - |
Gallus gallus | 5beta-androstan-17one-3-ol + NAD(P)+ | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 1.1.1.50 | ? | ? * 66000, SDS-PAGE | Gallus gallus |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 1.1.1.50 | 50 | - |
- |
Gallus gallus |
Cofactor
| EC Number | Cofactor | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.1.1.50 | NADPH | preference for | Gallus gallus | |
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Release 2023.1 (January 2023)
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