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Literature summary extracted from
- Enzymatic repair of pyrimidine dimer-containing DNA. A 5' dimer DNA glycosylase: 3'-apyrimidinic endonuclease mechanism from Micrococcus luteus (1982), J. Biol. Chem., 257, 13465-13474.
General Stability
| EC Number | General Stability | Organism |
|---|---|---|
| 3.2.2.17 | resistant to EDTA | Micrococcus luteus |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 4.2.99.18 | 4-chloromercuribenzoate | complete inhibition at 1 mM | Bacillus subtilis |  |
| 4.2.99.18 | EDTA | 96% inhibition at 20 mM | Bacillus subtilis | |
| 4.2.99.18 | NaCl | complete inhibition at 1 M, 40% inhibition at 0.5 M | Bacillus subtilis | |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.2.2.17 | 0.000015 | - |
DNA | thymine labeled DNA | Micrococcus luteus | |
| 3.2.2.17 | 0.00028 | - |
DNA | cytosine labeled DNA | Micrococcus luteus | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 3.2.2.17 | 17500 | - |
SDS-PAGE | Micrococcus luteus |
| 3.2.2.17 | 18000 | - |
- |
Micrococcus luteus |
| 4.2.99.18 | 26000 | - |
4 * 26000, SDS-PAGE | Bacillus subtilis |
| 4.2.99.18 | 105000 | - |
glycerol gradient centrifugation | Bacillus subtilis |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.2.2.17 | additional information | Micrococcus luteus | only nicking of UV-irradiated DNA, creating phospho diester bond breaks 5' with respect to pyrimidine dimers, protein containing the glycosylase activity also contains the apyrimidinic endonuclease activity, multifunctional enzyme, 2 distinct domains in a single enzyme molecule possess both pyrimidine dimer-DNA glycosylase, and AP endonuclease activity, first the glycosylase cleaves glycosylic bond of one of the pyrimidine residues of a dimer to yield an AP site, then AP endonuclease hydrolyzes the phospho diester bond near the AP site, prefers duplex DNA to single-stranded | ? | - |
? | |
| 4.2.99.18 | DNA | Bacillus subtilis | - |
fragments of DNA | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.2.2.17 | Micrococcus luteus | - |
- |
- |
| 4.2.99.18 | Bacillus subtilis | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.2.2.17 | - |
Micrococcus luteus |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 4.2.99.18 | additional information | - |
- |
Bacillus subtilis |
Storage Stability
| EC Number | Storage Stability | Organism |
|---|---|---|
| 3.2.2.17 | stable on ice for 8 months with no loss in activity | Micrococcus luteus |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.2.2.17 | DNA phiX174 RFI + H2O | irradiated with increasing doses of UV light | Micrococcus luteus | thymine + ? | - |
? | |
| 3.2.2.17 | additional information | only nicking of UV-irradiated DNA, creating phospho diester bond breaks 5' with respect to pyrimidine dimers, protein containing the glycosylase activity also contains the apyrimidinic endonuclease activity, multifunctional enzyme, 2 distinct domains in a single enzyme molecule possess both pyrimidine dimer-DNA glycosylase, and AP endonuclease activity, first the glycosylase cleaves glycosylic bond of one of the pyrimidine residues of a dimer to yield an AP site, then AP endonuclease hydrolyzes the phospho diester bond near the AP site, prefers duplex DNA to single-stranded | Micrococcus luteus | ? | - |
? | |
| 3.2.2.17 | oligonucleotides + H2O | - |
Micrococcus luteus | dCMP + dTMP | - |
? | |
| 4.2.99.18 | DNA | - |
Bacillus subtilis | fragments of DNA | - |
? | |
| 4.2.99.18 | DNA | hydrolyzes phosphodiester bond near heat-induced apruinic sites in double or single strand DNA | Bacillus subtilis | fragments of DNA | - |
? | |
| 4.2.99.18 | additional information | no substrate: alkylated sites | Bacillus subtilis | ? | - |
? | |
| 4.2.99.18 | additional information | no substrate: native DNA | Bacillus subtilis | ? | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 3.2.2.17 | monomer | 1 * 17500-18000, single polypeptide chain | Micrococcus luteus |
| 4.2.99.18 | tetramer | 4 * 26000, SDS-PAGE | Bacillus subtilis |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 3.2.2.17 | 5'dimer DNA glycosylase | - |
Micrococcus luteus |
Temperature Stability [°C]
| EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.2.2.17 | 45 | 55 | thermal inactivation of both endonuclease and glycosylase activity | Micrococcus luteus |
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