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Literature summary extracted from

  • Arluison, V.; Hountondji, C.; Robert, B.; Grosjean, H.
    Transfer RNA-Pseudouridine Synthetase Pus1 of Saccharomyces cerevisiae contains one atom of zinc essential for its native conformation and tRNA recognition (1998), Biochemistry, 37, 7268-7276.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.2.1.70
-
 Saccharomyces cerevisiae
5.4.99.B22 expression in Escherichia coli Saccharomyces cerevisiae

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.2.1.70 1,10-phenanthroline loss of activity within 5 min in the presence of 40 mM pseudouridine synthetase Pus1 Saccharomyces cerevisiae
4.2.1.70 thiol reagents
-
 Saccharomyces cerevisiae

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.2.1.70 0.000425
-
 tRNAVal Pus1, yeast tRNAVal Saccharomyces cerevisiae

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
4.2.1.70 Zn2+ essential for its native conformation and tRNA recognition Saccharomyces cerevisiae
5.4.99.B22 Zinc contains one atom of zinc per 63000 Da monomer. This zinc ion could not be removed by treatment with EDTA or urea. However, a zinc-depleted enzyme is obtained after prolonged dialysis against the specific chelating agent 1,10-phenanthroline. Removal of the zinc ion results in inactivation of the enzyme with concomitant loss of its ability to bind tRNA. Structural role for the zinc in the tRNA-pseudouridine synthetase Pus1 Saccharomyces cerevisiae

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.2.1.70 63200
-
 1 * 63200, MALDI mass spectrometry Saccharomyces cerevisiae

Organism

EC Number Organism UniProt Comment Textmining
4.2.1.70 Saccharomyces cerevisiae
-
-
-
5.4.99.B22 Saccharomyces cerevisiae Q12211
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
5.4.99.B22 recombinant enzyme Saccharomyces cerevisiae

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.2.1.70 additional information
-
-
 Saccharomyces cerevisiae

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.2.1.70 tRNAVal
-
 Saccharomyces cerevisiae ?
-
 ?

Subunits

EC Number Subunits Comment Organism
4.2.1.70 monomer 1 * 63200, MALDI mass spectrometry Saccharomyces cerevisiae

Synonyms

EC Number Synonyms Comment Organism
5.4.99.B22 PUS1
-
 Saccharomyces cerevisiae