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Literature summary extracted from
- Studies on the biosynthesis of 5-ribosyluracil 5'-monophosphate in Tetrahymena pyriformis (1964), J. Biol. Chem., 239, 1177-1187.
General Stability
| EC Number | General Stability | Organism |
|---|---|---|
| 4.2.1.70 | activity retained after freezing and thawing | Tetrahymena pyriformis |
| 4.2.1.70 | buffers containing 20% glycerol stabilize the enzyme | Tetrahymena pyriformis |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 4.2.1.70 | 0.024 | - |
ribose 5-phosphate | - |
Tetrahymena pyriformis |  |
| 4.2.1.70 | 0.071 | - |
Uracil | - |
Tetrahymena pyriformis | |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 4.2.1.70 | Mg2+ | required, broad maximal activity between 0.01 M and 0.05 M | Tetrahymena pyriformis | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 4.2.1.70 | Tetrahymena pyriformis | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 4.2.1.70 | partial | Tetrahymena pyriformis |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 4.2.1.70 | 13.2 | - |
- |
Tetrahymena pyriformis |
Storage Stability
| EC Number | Storage Stability | Organism |
|---|---|---|
| 4.2.1.70 | -20°C, stable for 1 year without loss of activity | Tetrahymena pyriformis |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 4.2.1.70 | 2-thiouracil + D-ribose 5-phosphate | - |
Tetrahymena pyriformis | ? | - |
? | |
| 4.2.1.70 | isocytosine + D-ribose 5-phosphate | - |
Tetrahymena pyriformis | ? | - |
? | |
| 4.2.1.70 | uracil + D-ribose 5-phosphate | - |
Tetrahymena pyriformis | pseudouridine 5'-phosphate + H2O | - |
? | |
| 4.2.1.70 | uracil + ribose 5-phosphate | r | Tetrahymena pyriformis | 5-ribosyluracil 5'-monophosphate + H2O | - |
? | |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 4.2.1.70 | 30 | - |
- |
Tetrahymena pyriformis |
Temperature Range [°C]
| EC Number | Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 4.2.1.70 | 24 | 37 | about 50% of maximal activity at 25°C and about 30% of maximal activity at 37°C | Tetrahymena pyriformis |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 4.2.1.70 | 8 | 8.5 | - |
Tetrahymena pyriformis |
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Release 2023.1 (January 2023)
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