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Literature summary extracted from

  • Yanase, H.; Sakai, T.; Tonomura, K.
    Metabolism of nitriles in microorganisms. Part V. Purification, crystallization and some properties of beta-cyano-L-alanine-degrading enzyme in Pseudomonas sp. 13 (1983), Agric. Biol. Chem., 47, 473-482.
No PubMed abstract available

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
3.5.5.4 crystallization in presence of (NH4)2SO4 Pseudomonas sp.
4.2.1.65
-
 Pseudomonas sp.

General Stability

EC Number General Stability Organism
3.5.5.4 2-mercaptoethanol stabilizes Pseudomonas sp.
4.2.1.65 very unstable, dithiothreitol, GSH and 2-mercaptoethanol protect Pseudomonas sp.

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.5.5.4 1,10-phenanthroline weak, 2-mercaptoethanol partially protects Pseudomonas sp.
3.5.5.4 2,2'-dipyridyl 2-mercaptoethanol partially protects Pseudomonas sp.
3.5.5.4 Ag2+ 2-mercaptoethanol partially protects Pseudomonas sp.
3.5.5.4 Cd2+ 2-mercaptoethanol partially protects Pseudomonas sp.
3.5.5.4 Co2+ 2-mercaptoethanol partially protects Pseudomonas sp.
3.5.5.4 Cu2+ 2-mercaptoethanol partially protects Pseudomonas sp.
3.5.5.4 Hg2+ 2-mercaptoethanol partially protects Pseudomonas sp.
3.5.5.4 iodoacetamide 2-mercaptoethanol partially protects Pseudomonas sp.
3.5.5.4 Mersalyl acid strong Pseudomonas sp.
3.5.5.4 Mg2+ weak Pseudomonas sp.
3.5.5.4 N-ethylmaleimide 2-mercaptoethanol partially protects Pseudomonas sp.
3.5.5.4 NaF 2-mercaptoethanol partially protects Pseudomonas sp.
3.5.5.4 NaNO3 2-mercaptoethanol partially protects Pseudomonas sp.
3.5.5.4 Ni2+ weak Pseudomonas sp.
3.5.5.4 p-chloromercuribenzoate strong Pseudomonas sp.
3.5.5.4 p-chloromercuriphenylsulfonic acid strong Pseudomonas sp.
4.2.1.65 2,2'-dipyridyl poor Pseudomonas sp.
4.2.1.65 Ag+ the inhibitory effect of metal ions is decreased in presence of 2-mercaptoethanol Pseudomonas sp.
4.2.1.65 Cd2+ the inhibitory effect of metal ions is decreased in presence of 2-mercaptoethanol Pseudomonas sp.
4.2.1.65 Cu2+ the inhibitory effect of metal ions is decreased in presence of 2-mercaptoethanol Pseudomonas sp.
4.2.1.65 HgCl2 the inhibitory effect of metal ions is decreased in presence of 2-mercaptoethanol Pseudomonas sp.
4.2.1.65 iodoacetic acid poor Pseudomonas sp.
4.2.1.65 Mersalyl acid
-
 Pseudomonas sp.
4.2.1.65 N-ethylmaleimide poor Pseudomonas sp.
4.2.1.65 NaF poor Pseudomonas sp.
4.2.1.65 NaNO2 poor Pseudomonas sp.
4.2.1.65 p-chloromercuribenzoate
-
 Pseudomonas sp.
4.2.1.65 p-chloromercuriphenyl sulfonic acid
-
 Pseudomonas sp.

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.5.5.4 83
-
 3-Cyano-L-alanine pH 9.5, 37°C Pseudomonas sp.
4.2.1.65 83
-
 Beta-cyano-L-alanine
-
 Pseudomonas sp.

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.5.5.4 35000
-
 30 * 35000, SDS-PAGE Pseudomonas sp.
3.5.5.4 1000000
-
 ultracentrifugation Pseudomonas sp.
4.2.1.65 35000
-
 x * 35000, SDS-PAGE Pseudomonas sp.
4.2.1.65 1000000
-
 gel filtration Pseudomonas sp.

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.5.5.4 3-cyano-L-alanine + H2O Pseudomonas sp.
-
 L-aspartate + NH3 L-asparagine is formed as an intermediate ?
3.5.5.4 3-cyano-L-alanine + H2O Pseudomonas sp. 13
-
 L-aspartate + NH3 L-asparagine is formed as an intermediate ?
4.2.1.65 beta-Cyano-L-alanine + H2O Pseudomonas sp. cyanide assimilation ?
-
 ?
4.2.1.65 beta-Cyano-L-alanine + H2O Pseudomonas sp. 13 cyanide assimilation ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.5.5.4 Pseudomonas sp.
-
-
-
3.5.5.4 Pseudomonas sp. 13
-
-
-
4.2.1.65 Pseudomonas sp.
-
 13
-
4.2.1.65 Pseudomonas sp. 13
-
 13
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.5.5.4
-
 Pseudomonas sp.
4.2.1.65
-
 Pseudomonas sp.

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.5.5.4 65
-
-
 Pseudomonas sp.
4.2.1.65 54.7
-
-
 Pseudomonas sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.5.4 3-cyano-L-alanine + H2O
-
 Pseudomonas sp. L-aspartate + NH3 L-asparagine is formed as an intermediate ?
3.5.5.4 3-cyano-L-alanine + H2O
-
 Pseudomonas sp. 13 L-aspartate + NH3 L-asparagine is formed as an intermediate ?
3.5.5.4 additional information no substrates are various nitrile or amide compounds, overview Pseudomonas sp. ?
-
 ?
3.5.5.4 additional information no substrates are various nitrile or amide compounds, overview Pseudomonas sp. 13 ?
-
 ?
4.2.1.65 beta-Cyano-L-alanine + H2O highly specific for beta-cyano-L-alanine Pseudomonas sp. L-Asparagine
-
 ?
4.2.1.65 beta-Cyano-L-alanine + H2O highly specific for beta-cyano-L-alanine Pseudomonas sp. 13 L-Asparagine
-
 ?
4.2.1.65 beta-Cyano-L-alanine + H2O cyanide assimilation Pseudomonas sp. ?
-
 ?
4.2.1.65 beta-Cyano-L-alanine + H2O cyanide assimilation Pseudomonas sp. 13 ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.5.5.4 polymer 30 * 35000, SDS-PAGE Pseudomonas sp.
4.2.1.65 ? x * 35000, SDS-PAGE Pseudomonas sp.

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.5.5.4 55
-
-
 Pseudomonas sp.
4.2.1.65 55
-
-
 Pseudomonas sp.

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.5.5.4 30 62 about half-maximal activity at 30°C and 62°C Pseudomonas sp.
4.2.1.65 20 65
-
 Pseudomonas sp.

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.5.5.4 60
-
 stable up to for 10 min in 50 mM phosphate buffer, pH 7.5 Pseudomonas sp.
4.2.1.65 60
-
 up to, 10 min Pseudomonas sp.

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.5.5.4 9.5
-
 Tris-HCl buffer Pseudomonas sp.
4.2.1.65 9.5
-
-
 Pseudomonas sp.

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.5.5.4 8.2 10.8 about half-maximal activity at pH 8.2 and pH 10.8 Pseudomonas sp.
4.2.1.65 7 11
-
 Pseudomonas sp.

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.5.5.4 7.5
-
 3 h, stable at 37°C, only in the presence of 2-mercaptoethanol Pseudomonas sp.
4.2.1.65 7 11 in presence of 2-mercaptoethanol Pseudomonas sp.