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Literature summary extracted from

  • Topham, R.W.; Gaylor, J.L.
    Isolation and purification of 5alpha-hydroxysterol dehydrase of yeast (1970), J. Biol. Chem., 245, 2319-2327.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
4.2.1.62 additional information no requirement Saccharomyces cerevisiae

Organism

EC Number Organism UniProt Comment Textmining
4.2.1.62 Saccharomyces cerevisiae
-
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
4.2.1.62 side-chain modification phospholipid associated with active enzyme, removal by digestion with phospholipase A does not reduce the activity Saccharomyces cerevisiae

Purification (Commentary)

EC Number Purification (Comment) Organism
4.2.1.62
-
 Saccharomyces cerevisiae

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.2.1.62 0.121
-
-
 Saccharomyces cerevisiae

Storage Stability

EC Number Storage Stability Organism
4.2.1.62 -20°C, 0.4 M potassium phosphate buffer, pH 7.4, 10 mM 2-mercaptoethanol, 2 weeks, no loss of activity Saccharomyces cerevisiae

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.2.1.62 5alpha-Ergosta-7,22-diene-3beta,5alpha-diol
-
 Saccharomyces cerevisiae Ergosterol + H2O
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
4.2.1.62 37
-
 enzyme assay at Saccharomyces cerevisiae

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.2.1.62 7.4
-
 enzyme assay at Saccharomyces cerevisiae